HAUSP / USP7 protein (Tagged-His Tag) (ab91746)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium hydrogen phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Deubiquitinating enzyme 7Herpes virus associated ubiquitin specific proteaseHerpesvirus-associated ubiquitin-specific protease
- TEF 1tef-1TEF1Ubiquitin carboxyl terminal hydrolase 7Ubiquitin carboxyl-terminal hydrolase 7Ubiquitin specific peptidase 7Ubiquitin specific peptidase 7 (herpes virus associated)Ubiquitin specific peptidase 7 herpes virus associatedUbiquitin specific processing protease 7Ubiquitin specific protease 7Ubiquitin specific protease 7 herpes virus associatedUbiquitin thioesterase 7Ubiquitin thiolesterase 7Ubiquitin-specific-processing protease 7UBP 7UBP-7UBP7UBP7_HUMANUSP 7usp-7USP7VMW110-ASSOCIATED PROTEIN, 135-KD
Contains 1 MATH domain.
modificationsIsoform 1: Phosphorylated. Isoform 1 is phosphorylated at positions Ser-18 and Ser-963. Isoform 2: Not phosphorylated.
Isoform 1: Polyneddylated. Isoform 2: Not Polyneddylated.
Isoform 1 and isoform 2: Not sumoylated.
Isoform 1 and isoform 2: Polyubiquitinated by herpesvirus 1 trans-acting transcriptional protein ICP0/VMW110; leading to its subsequent proteasomal degradation. Isoform 1: Ubiquitinated at Lys-869.
HAUSP / USP7 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for HAUSP / USP7 protein (Tagged-His Tag) (ab91746)
ab91746 has not yet been referenced specifically in any publications.