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I'm trying to detect the different isoforms of the rat protein. What were the immunogens for these antibodies? Will they cross-react with the different isoforms? |
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ANSWER: |
Thank you for your inquiry. Here is the alignment for the immunogen for both ab78939 and ab79723 (they are raised against the same peptide): The isoforms and the percentage of overlap with the immunogen: http://www.uniprot.org/uniprot/Q7TSE9#section_alternative Cat. No. ab78939 and ab79723 Q7TSE9-1 100% Q7TSE9-2 100% Q7TSE9-3 75% [ .************] Q7TSE9-5 25% [------------****] Q7TSE9-6 25% [------------****] If it's greater than 84%, it will most likely cross-react. I hope this information helps. Please contact us with any other questions. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Lane 1 : Anti-HAX1 antibody [9G3D11] (ab79723) at 1 µg/ml
Lane 2 : Anti-HAX1 antibody [9G3D11] (ab79723) at 2 µg/ml
Lane 1 : Human brain tissue lysate
Lane 2 : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 32 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
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