Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Cell Biology >> Apoptosis >> Mitochondrial
Overview
Product name
Anti-HAX1 antibody
See all HAX1 products (4) ...
Description
Goat polyclonal to HAX1
Specificity
ab81973 is expected to recognize both reported isoforms (NP_006109.2 and NP_001018238.1).
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: C-TRHEADSSPRGDPES, corresponding to internal sequence amino acids 241-255 of human HAX1 according to NP_006109.2 (or 193-207 of NP_001018238.1).
C-TRHEADSS PRGDPES
Positive control
Human kidney, liver and testis lysates.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Cell Biology >> Apoptosis >> Mitochondrial
-
Western blot - HAX1 antibody (ab81973)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab81973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
Peptide ELISA: antibody detection limit dilution 1:2,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Relevance
HAX1 associates with HS1, binding to its N-terminal region. It is also known to associate with PKD2 (involved in polycystic kidney disease) and with cortactin/EMS1. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.
Cellular localization
Endoplasmic reticulum, Mitochondrial and Nuclear membrane. Note: Predominantly mitochondrial, but also to a lesser extent in endoplasmic reticulum and nuclear envelope.
Alternative names
- FLJ17042 antibody
- FLJ18492 antibody
- FLJ93803 antibody
see all
Database links
- Entrez Gene: 10456 Human
- Omim: 605998 Human
- SwissProt: O00165 Human
- Unigene: 199625 Human
Anti-HAX1 antibody images:
Western blot - HAX1 antibody (ab81973)
Anti-HAX1 antibody (ab81973) at 1 µg/ml + human testis lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 1 hour
References for Anti-HAX1 antibody (ab81973)
ab81973 has not yet been referenced specifically in any publications.
Publishing research using ab81973? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"