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Western blot - HAX1 antibody (ab81973)
(enlarge)
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Product Name
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HAX1 antibody
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See all HAX1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to HAX1
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Immunogen
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Synthetic peptide: C-TRHEADSSPRGDPES, corresponding to internal sequence amino acids 241-255 of human HAX1 according to NP_006109.2 (or 193-207 of NP_001018238.1).
BLAST 'C-TRHEADSSPRGDPES' with  or 
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Reacts with
(species key)
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Hu
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Specificity
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ab81973 is expected to recognize both reported isoforms (NP_006109.2 and NP_001018238.1).
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:2,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human kidney, liver and testis lysates.
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Cellular localization
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Endoplasmic reticulum, Mitochondrial and Nuclear membrane. Note: Predominantly mitochondrial, but also to a lesser extent in endoplasmic reticulum and nuclear envelope.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Cell Biology >> Apoptosis >> Mitochondrial
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Relevance
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HAX1 associates with HS1, binding to its N-terminal region. It is also known to associate with PKD2 (involved in polycystic kidney disease) and with cortactin/EMS1. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HAX1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HAX1 antibody images, references, products related to ab81973 and other tools.
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HAX1 antibody images:
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 Western blot - HAX1 antibody (ab81973)
HAX1 antibody (ab81973) at 1 µg/ml + human testis lysate in RIPA buffer at 35 µg
developed using the ECL technique
Predicted band size : 32 kDa
Observed band size : 32 kDa
Exposure time : 1 hour
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Search PubMed (MEDLINE) for references to HAX1
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HAX1 antibody - more information
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Customer reviews (feedback) regarding HAX1 antibody |
Customer FAQs regarding HAX1 antibody |
Protocols for HAX1 antibody |
Price and availability of products related to HAX1 antibody |
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HAX1 antibody - related products:
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HAX1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HAX1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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