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Western blot - HAX1 antibody (ab81973)
Western blot - HAX1 antibody (ab81973)
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Product Name 

HAX1 antibody

 

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Product type 

Primary antibodies

Description 

Goat polyclonal to HAX1

Immunogen 

Synthetic peptide: C-TRHEADSSPRGDPES, corresponding to internal sequence amino acids 241-255 of human HAX1 according to NP_006109.2 (or 193-207 of NP_001018238.1).
BLAST 'C-TRHEADSSPRGDPES' with BLAST the sequence with ExPASy or BLAST the sequence with NCBI

Reacts with 
(species key)

Hu

Specificity 

ab81973 is expected to recognize both reported isoforms (NP_006109.2 and NP_001018238.1).

Tested applications 
(see key)

ELISA, WB


Abreviews 

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(see key)


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Application notes 
(see key)

Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:2,000.
WB: Use at a concentration of 1 - 3 µg/ml. Predicted molecular weight: 32 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human kidney, liver and testis lysates.

Cellular localization 

Endoplasmic reticulum, Mitochondrial and Nuclear membrane. Note: Predominantly mitochondrial, but also to a lesser extent in endoplasmic reticulum and nuclear envelope.

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Cell Biology >> Apoptosis >> Mitochondrial

Relevance 

HAX1 associates with HS1, binding to its N-terminal region. It is also known to associate with PKD2 (involved in polycystic kidney disease) and with cortactin/EMS1. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.

 

HAX1 antibody (ab81973)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

10456    (Human)

Omim

    

605998    (Human)

SwissProt

    

O00165    (Human)

Unigene

    

199625    (Human)

Raised in 

Goat

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris saline, pH 7.3

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Purification notes 

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HAX1 antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for HAX1 antibody images, references, products related to ab81973 and other tools.

 

HAX1 antibody images:

Product image  Western blot - HAX1 antibody (ab81973)

Western blot - HAX1 antibody (ab81973)

HAX1 antibody (ab81973) at 1 µg/ml + human testis lysate in RIPA buffer at 35 µg
developed using the ECL technique

Predicted band size : 32 kDa
Observed band size : 32 kDa


Exposure time : 1 hour

 
 

Search PubMed (MEDLINE) for references to HAX1

 

HAX1 antibody - more information

Customer reviews (feedback) regarding HAX1 antibody

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HAX1 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for HAX1 antibody

ab14836

  

Liver (Human) Nuclear Lysate

ab29884

  

Liver (Human) Nuclear Lysate - adult normal tissue

ab30198

  

Kidney (Human) Nuclear Lysate - adult normal tissue

ab30253

  

Testis (Human) Nuclear Lysate - adult normal tissue

ab30258

  

Testis (Human) Whole Cell Lysate - tumor tissue

 

Compatible Secondaries for HAX1 antibody

ab6566

  

Cy5 ® antibody

ab6739

  

Texas Red ® antibody

ab6740

  

Biotin antibody

ab6741

  

HRP antibody

ab6742

  

Alkaline Phosphatase antibody

ab6881

  

FITC antibody

ab7004

  

Phycoerythrin antibody

ab27245

  

10nm Gold antibody

ab27246

  

10nm Gold antibody

ab27247

  

15nm Gold antibody

 

Other HAX1 Antibodies

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HAX1 antibody - other tools:

Contact Abcam with a Technical Enquiry about HAX1 antibody

 

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