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Products:Cell Biology >> Apoptosis >> Mitochondrial
Overview
Product name
Anti-HAX1 antibody
See all HAX1 products (4) ...
Description
Rabbit polyclonal to HAX1
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 108-157 (PGPESETPGE RLREGQTLRD SMLKYPDSHQ PRIFGGVLES DARSESPQPA) of Human HAX1 (NP_006109).
PGPESETPGE RLREGQTLRD SMLKYPDSHQ PRIFGGVLES DARSESPQPA
Positive control
COLO205 cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Cell Biology >> Apoptosis >> Mitochondrial
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Western blot - HAX1 antibody (ab87185)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 32 kDa.
Target
Relevance
HAX1 associates with HS1, binding to its N-terminal region. It is also known to associate with PKD2 (involved in polycystic kidney disease) and with cortactin/EMS1. HAX1 is also reported to bind to hairpin structures in vimentin and DNA polymerase beta mRNAs, so may play a role in mRNA stability and transport. It may also function in promoting cell survival. Defects in HAX1 are the cause of autosomal recessive severe congenital neutropenia 3 (SCN3) also called Kostmann disease.
Cellular localization
Endoplasmic reticulum, Mitochondrial and Nuclear membrane. Note: Predominantly mitochondrial, but also to a lesser extent in endoplasmic reticulum and nuclear envelope.
Alternative names
- FLJ17042 antibody
- FLJ18492 antibody
- FLJ93803 antibody
see all
Database links
- Entrez Gene: 10456 Human
- Entrez Gene: 23897 Mouse
- Entrez Gene: 291202 Rat
- Omim: 605998 Human
- SwissProt: O00165 Human
- SwissProt: O35387 Mouse
- SwissProt: Q7TSE9 Rat
- Unigene: 199625 Human
Anti-HAX1 antibody images:
Western blot - HAX1 antibody (ab87185)
Anti-HAX1 antibody (ab87185) at 1 µg/ml (in 5% skim milk / PBS buffer) + COLO205 cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 32 kDa
Observed band size : 30 kDa (why is the actual band size different from the predicted?)
Additional bands at : 50 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Gel concentration: 12%
References for Anti-HAX1 antibody (ab87185)
ab87185 has not yet been referenced specifically in any publications.
Publishing research using ab87185? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"