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If your product does not perform as described on this datasheet, we will refund or replace your product...
Read our guarantee »This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab66792 for help.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
![Western blot - HB EGF antibody [4G10] - Azide free (ab66792)](/ps/datasheet/Images/66/ab66792/ab66791wb-1.jpg)
Western blot - HB EGF antibody [4G10] - Azide free (ab66792)
All lanes : Anti-HB EGF antibody [4G10] - Azide free (ab66792) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HB-EGF expression vector
Predicted band size : 23 kDa
Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
![Western blot - HB EGF antibody [4G10] - Azide free (ab66792)](/ps/datasheet/Images/66/ab66792/ab66792c.jpg)
Western blot - HB EGF antibody [4G10] - Azide free (ab66792)
All lanes : Anti-HB EGF antibody [4G10] - Azide free (ab66792) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HB-EGF expression vector
Predicted band size : 23 kDa
Observed band size : 20-38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
This image shows Immunoprecipitation of HB EGF followed by Western blotting; the cell surface was biotinylated
![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)](/ps/datasheet/Images/66/ab66792/ab66792ic.jpg)
Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)
These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
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