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Western blot - HB EGF antibody [4G10] - Azide free (ab66792)
![Western blot - HB EGF antibody [4G10] - Azide free (ab66792)](/ps/datasheet/images/ab66791wb-1.jpg)
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Western blot - HB EGF antibody [4G10] - Azide free (ab66792)
![Western blot - HB EGF antibody [4G10] - Azide free (ab66792)](/ps/datasheet/images/ab66792c.jpg)
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Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)
![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)](/ps/datasheet/images/ab66792ic.jpg)
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Product Name
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HB EGF antibody [4G10] - Azide free
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See all HB EGF antibodies (4)...
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [4G10] to HB EGF - Azide free
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Immunogen
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Recombinant human HB EGF ectodomain expressed in SF21 cells.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Epitope
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EGF domain
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Tested applications
(see key)
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ICC/IF, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 5 - 10 µg/ml.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Vero cell extract carrrying human HB-EGF expression vector.
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Cellular localization
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Type I membrane protein. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
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Research areas
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Signal Transduction >> Growth Factors/Hormones >> EGF
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Relevance
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Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a 22kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or Nterminal truncation. HB-EGF is synthesized as a membrane-anchored precursor(proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively.HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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4G10
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Isotype
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IgG1
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Purity
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Protein G purified
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Storage buffer
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Preservative: None
Constituents: 50% Glycerol, PBS Material safety datasheet (MSDS) for this product:
Glycerol MSDS
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Purification notes
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Produced in serum-free medium and purified by combination of chromatography
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HB EGF antibody [4G10] - Azide free is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HB EGF antibody [4G10] - Azide free images, references, products related to ab66792 and other tools.
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HB EGF antibody [4G10] - Azide free images:
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 Western blot - HB EGF antibody [4G10] - Azide free (ab66792)
All lanes : HB EGF antibody [4G10] - Azide free (ab66792) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HB-EGF expression vector
Predicted band size : 23 kDa
Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
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Western blot - HB EGF antibody [4G10] - Azide free (ab66792)
All lanes : HB EGF antibody [4G10] - Azide free (ab66792) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HB-EGF expression vector
Predicted band size : 23 kDa
Observed band size : 20-38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
This image shows Immunoprecipitation of HB EGF followed by Western blotting; the cell surface was biotinylated
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Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)
These images show Vero cells carrying
human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
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Search PubMed (MEDLINE) for references to HB EGF
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HB EGF antibody [4G10] - Azide free - more information
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Customer reviews (feedback) regarding HB EGF antibody [4G10] - Azide free |
Customer FAQs regarding HB EGF antibody [4G10] - Azide free |
Protocols for HB EGF antibody [4G10] - Azide free |
Price and availability of products related to HB EGF antibody [4G10] - Azide free |
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HB EGF antibody [4G10] - Azide free - related products:
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HB EGF antibody [4G10] - Azide free - other tools:
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Contact Abcam with a Technical Enquiry about HB EGF antibody [4G10] - Azide free |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Western blot - HB EGF antibody [4G10] - Azide free (ab66792)](/index.html?datasheet=66792#ab66791wb-1.jpg)
![Western blot - HB EGF antibody [4G10] - Azide free (ab66792)](/index.html?datasheet=66792#ab66792c.jpg)
![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)](/index.html?datasheet=66792#ab66792ic.jpg)
