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Anti-HB EGF antibody [4G10] - Azide free (ab66792)

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Overview

Product name

Anti-HB EGF antibody [4G10] - Azide free
See all HB EGF products (5) ...

Description

Mouse monoclonal [4G10] to HB EGF - Azide free

Tested applications

IP, WB, ICC/IFmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse

Immunogen

Recombinant human HB EGF ectodomain expressed in SF21 cells.

Epitope

EGF domain

Positive control

Vero cell extract carrrying human HB-EGF expression vector.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 50% Glycerol, PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Produced in serum-free medium and purified by combination of chromatography

Clonality

Monoclonal

Clone number

4G10

Isotype

IgG1

Research areas

Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF

  • Western blot - HB EGF antibody [4G10] - Azide free (ab66792)Western blot - HB EGF antibody [4G10] - Azide free (ab66792) image (enlarge)

  • Western blot - HB EGF antibody [4G10] - Azide free (ab66792)Western blot - HB EGF antibody [4G10] - Azide free (ab66792) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab66792 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.

Sequence similarities

Contains 1 EGF-like domain.

Post-translational
modifications

Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).

Cellular localization

Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.

Target information above from: UniProt accessionQ99075 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Diphtheria toxin receptor antibody
  • DT R antibody
  • DT-R antibody
  • DTR antibody
  • DTS antibody
  • DTSF antibody
  • HB-EGF antibody
  • HBEGF antibody
  • HBEGF_HUMAN antibody
  • HEGFL antibody
  • Heparin binding EGF like growth factor antibody
  • Heparin binding epidermal growth factor antibody
  • Heparin binding epidermal growth factor like growth factor antibody
  • Heparin-binding EGF-like growth factor antibody
see all

Database links

Anti-HB EGF antibody [4G10] - Azide free images:

  Western blot - HB EGF antibody [4G10] - Azide free (ab66792)

Western blot - HB EGF antibody [4G10] - Azide free (ab66792)

All lanes : Anti-HB EGF antibody [4G10] - Azide free (ab66792) at 1 µg/ml

Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector


Predicted band size : 23 kDa
Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

  Western blot - HB EGF antibody [4G10] - Azide free (ab66792)

Western blot - HB EGF antibody [4G10] - Azide free (ab66792)

All lanes : Anti-HB EGF antibody [4G10] - Azide free (ab66792) at 1 µg/ml

Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector


Predicted band size : 23 kDa
Observed band size : 20-38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.

This image shows Immunoprecipitation of HB EGF followed by Western blotting; the cell surface was biotinylated

  Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)

Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] - Azide free (ab66792)

These images show Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab 66792, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.

References for Anti-HB EGF antibody [4G10] - Azide free (ab66792)

ab66792 has not yet been referenced specifically in any publications.

Publishing research using ab66792? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"