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Anti-HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)

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If your product does not perform as described on this datasheet, we will refund or replace your product...

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This product is covered by the Abpromise guarantee. Our scientific support team are available to answer any questions or queries - fill out an inquiry form for ab66793 for help.

Alternatively, you can search the previous enquiries about this product to see if your query has already been answered.

2 questions for ab66793

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Question 1

Monday 19-September-2011

Is it possible to use both a biotin- and a mouse-specific secondary antibody on ab66793?

 

ANSWER:

  

Thank you for your inquiry.

You probably could use an anti-mouse secondary antibody with ab66793 since we know it worked well with a Cy3-conjugated secondary antibody. But, there are 7.5 biotin molecules per IgG1 molecule, and that could cause a lot of hindrance to block the binding of the secondary antibody.

We do have anti-biotin conjugated antibodies (if that's what you are looking for), such as Anti-Biotin (Alkaline Phosphatase) ab6652, Anti-Biotin (HRP) ab19221, and Anti-Biotin (FITC) ab6650.

Click here (or use the following: http://www.abcam.com/index.html?datasheet=6652).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=19221).

Click here (or use the following: http://www.abcam.com/index.html?datasheet=6650).

What is probably best to do is to use a strepatvidin conjugate to bind the biotin on ab66793. We have two (peroxidase and AP).

http://www.abcam.com/Streptavidin-Peroxidase-Ready-to-Use-ab64269.html

http://www.abcam.com/Streptavidin-Alkaline-Phosphatase-Ready-to-Use-ab64268.html

I hope this information helps. Please contact us with any other questions.

Question 2

Thursday 27-August-2009

I have a question regarding ab66793. Does this antibody have cross-reactivity with Monkey, like Macaque?

ANSWER:

  

We could not find the sequence of Macaque monkey HB-EGF in databases. However, since the green monkey HB-EGF sequence has 97% identity to that of human, there is a good chance that ab66793 react with Macaque HB-EGF. Though the human HB-EGF has 80% identity with that of mouse and ab66793 did not react with mouse HB-EGF.

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