|
|
|
|
Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/ps/datasheet/images/ab66792ic.jpg)
(enlarge)
|
|
Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
![Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/ps/datasheet/images/ab66792c.jpg)
(enlarge)
|
|
Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
![Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/ps/datasheet/images/ab66791wb-1.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
HB EGF antibody [4G10] (Biotin) - Azide free
|
| |
See all HB EGF antibodies (4)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [4G10] to HB EGF - Azide free (Biotin)
|
|
Immunogen
|
Recombinant human HB EGF ectodomain expressed in SF21 cells.
|
|
Reacts with
|
Hu
|
|
Does not react with
(species key)
|
Ms
|
|
Epitope
|
EGF domain
|
|
Tested applications
(see key)
|
ICC/IF, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ICC/IF: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution.
WB: Use at a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
Vero cell extract carrying human HG-EGF expression vector.
|
|
Cellular localization
|
Type I membrane protein. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
|
|
Research areas
|
Signal Transduction >> Growth Factors/Hormones >> EGF
|
|
Relevance
|
Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a 22kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or Nterminal truncation. HB-EGF is synthesized as a membrane-anchored precursor(proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively.HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
4G10
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein G purified
|
|
Conjugation notes
|
biotin/IgG = 7.5
|
|
Storage buffer
|
Preservative: None
Constituents: 50% Glycerol, PBS Material safety datasheet (MSDS) for this product:
Glycerol MSDS
|
|
Purification notes
|
Produced in serum-free medium and purified by combination of chromatography.
|
|
Form
|
Liquid
|
|
Conjugation
|
Biotin
|
|
Concentration
|
0.600 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HB EGF antibody [4G10] (Biotin) - Azide free is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for HB EGF antibody [4G10] (Biotin) - Azide free images, references, products related to ab66793 and other tools.
|
| |
HB EGF antibody [4G10] (Biotin) - Azide free images:
|
 Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
|
| |
Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
All lanes : HB EGF antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml.
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector
Predicted band size : 23 kDa
Observed band size : 20-38 kDa
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
This image shows Immunoprecipitation of HB EGF followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
|
| |
Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
All lanes : HB EGF antibody [4G10] (Biotin) - Azide free (ab66793) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HB-EGF expression vector.
Predicted band size : 23 kDa
Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
|
| |
| |
|
Search PubMed (MEDLINE) for references to HB EGF
|
| |
HB EGF antibody [4G10] (Biotin) - Azide free - more information
|
Customer reviews (feedback) regarding HB EGF antibody [4G10] (Biotin) - Azide free |
Customer FAQs regarding HB EGF antibody [4G10] (Biotin) - Azide free |
Protocols for HB EGF antibody [4G10] (Biotin) - Azide free |
Price and availability of products related to HB EGF antibody [4G10] (Biotin) - Azide free |
| |
HB EGF antibody [4G10] (Biotin) - Azide free - related products:
|
|
|
| |
HB EGF antibody [4G10] (Biotin) - Azide free - other tools:
|
Contact Abcam with a Technical Enquiry about HB EGF antibody [4G10] (Biotin) - Azide free |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/index.html?datasheet=66793#ab66792ic.jpg)
![Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/index.html?datasheet=66793#ab66792c.jpg)
![Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/index.html?datasheet=66793#ab66791wb-1.jpg)
