![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793) image](#ab66792ic.jpg)
![Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793) image](#ab66792c.jpg)
![Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793) image](#ab66791wb-1.jpg)
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Products:Signal Transduction >> Growth Factors/Hormones >> EGF
Overview
Product name
Anti-HB EGF antibody [4G10] (Biotin) - Azide free
See all HB EGF products (5) ...
Description
Mouse monoclonal [4G10] to HB EGF - Azide free (Biotin)
Conjugation
Biotin
Conjugation notes
biotin/IgG = 7.5
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Recombinant human HB EGF ectodomain expressed in SF21 cells.
Epitope
EGF domain
Positive control
Vero cell extract carrying human HG-EGF expression vector.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Produced in serum-free medium and purified by combination of chromatography.
Clonality
Monoclonal
Clone number
4G10
Isotype
IgG1
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab66793 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ICC/IF: Use at an assay dependent dilution.
WB: Use a concentration of 0.2 - 1 µg/ml.Detects a band of approximately 20-28 kDa (predicted molecular weight: 23 kDa).
Target
Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Sequence similarities
Contains 1 EGF-like domain.
Post-translational
modifications
Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
Cellular localization
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
Target information above from: UniProt accessionQ99075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Diphtheria toxin receptor antibody
- DT R antibody
- DT-R antibody
see all
Database links
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
Anti-HB EGF antibody [4G10] (Biotin) - Azide free images:
Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
![Immunocytochemistry/ Immunofluorescence - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/ps/datasheet/Images/66/ab66793/ab66792ic.jpg)
These images shows Vero cells carrying human HG-EGF expression vector (left hand image) and Vero cells carrying mouse HB-EGF expression vector (right hand image). Cells treated with ab66793, fixed with 4% PFA and reacted with Cys3 conjugated 2nd antibody.
Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
![Immunoprecipitation - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/ps/datasheet/Images/66/ab66793/ab66792c.jpg)
All lanes : HB EGF antibody [4G10](biotin) - Azide free (ab66793) at 1 µg/ml. Lane 1 : Vero cell extract Lane 2 : Vero cell extract from cells carrrying human HB-EGF expression vector. Lane 3 : Vero cell extract from cells carrying mouse HB-EGF expression vector Predicted band size : 23 kDa Observed band size : 20-38 kDa Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands. This image shows Immunoprecipitation of HB EGF followed by Western blotting/SDS PAGE; the cell surface was biotinylated.
Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
![Western blot - HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)](/ps/datasheet/Images/66/ab66793/ab66791wb-1.jpg)
All lanes : Anti-HB EGF antibody [4G10] (Biotin) - Azide free (ab66793) at 1 µg/ml
Lane 1 : Vero cell extract
Lane 2 : Vero cell extract from cells
carrrying human HB-EGF expression vector.
Lane 3 : Vero cell extract from cells carrying
mouse HB-EGF expression vector.
Predicted band size : 23 kDa
Observed band size : 20-28 kDa (why is the actual band size different from the predicted?)
Additional bands at : 41 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HB EGF antibody [4G10] (Biotin) - Azide free (ab66793)
ab66793 has not yet been referenced specifically in any publications.
Publishing research using ab66793? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"