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Products:Signal Transduction >> Growth Factors/Hormones >> EGF
Overview
Product name
Anti-HB EGF antibody
See all HB EGF products (5) ...
Description
Goat polyclonal to HB EGF
Specificity
This antibody will neutralize the biological activity of recombinant human HB EGF. It will not neutralize the biological activity of EGF, TGFalpha, or amphiregulin.
Tested applications
ELISA, Neutralising, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
Recombinant full length protein, corresponding to amino acids 23-108 of Human HB EGF. The immunogen does not contain the pro-peptide.
Positive control
Vascular smooth muscle cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: <40% Trehalose, PBS, BSA
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified.
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Invasion/microenvironment >> Angiogenesis >> Angiogenic growth factors
Cancer >> Growth factors >> EGF
Signal Transduction >> Growth Factors/Hormones >> EGF
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.5 - 1 µg/ml.
Neut: Use at an assay dependent dilution. (3-6 ug/ml of antibody will neutralize 50% of the bioactivity.)
WB: Use a concentration of 1 - 2 µg/ml.Predicted molecular weight: 23 kDa.
Target
Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor.
Sequence similarities
Contains 1 EGF-like domain.
Post-translational
modifications
Several N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937).
Cellular localization
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
Target information above from: UniProt accessionQ99075
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Diphtheria toxin receptor antibody
- DT R antibody
- DT-R antibody
see all
Database links
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
References for Anti-HB EGF antibody (ab16783)
This product has been referenced in:
- Svensson KJet al. Hypoxia triggers a proangiogenic pathway involving cancer cell microvesicles and PAR-2-mediated heparin-binding EGF signaling in endothelial cells. Proc Natl Acad Sci U S A 108:13147-52 (2011). ICC/IF; Human.Read more (PubMed: 21788507) »
See 1 publication for this product
Publishing research using ab16783? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"