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Product Name
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HB EGF antibody
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See all HB EGF antibodies (3)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to HB EGF
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Immunogen
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Recombinant full length protein, corresponding to amino acids 23-108 of Human HB EGF. The immunogen does not contain the pro-peptide.
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Reacts with
(species key)
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Hu
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Specificity
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This antibody will neutralize the biological activity of recombinant human HB EGF. It will not neutralize the biological activity of EGF, TGFalpha, or amphiregulin.
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Tested applications
(see key)
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ELISA, Neut, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: Use at a concentration of 0.5 - 1 µg/ml.
Neut: 3-6 ug/ml of antibody will neutralize 50% of the bioactivity.
WB: Use at a concentration of 1 - 2 µg/ml. Predicted molecular weight: 23 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Vascular smooth muscle cells.
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Cellular localization
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Type I membrane protein. Mature HB-EGF is released into the extracellular space and probably binds to a receptor.
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Research areas
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Signal Transduction >> Growth Factors/Hormones >> EGF
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Relevance
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Heparin-binding epidermal growth factor-like growth factor (HB-EGF) is a 22kDa O-glycosylated protein that is a potent mitogen and chemoattractant for vascular smooth muscle cells, fibroblasts and epithelial cells but not endothelial cells. The natural protein has an apparent molecular mass of 19-23 kDa and exists in multiple forms as a result of heterogeneous O-glycosylation and/or Nterminal truncation. HB-EGF is synthesized as a membrane-anchored precursor(proHB-EGF) that is proteolytically cleaved to release the soluble mature growth factor. The two forms are active as juxtacrine and paracrine/autocrine growth factors respectively.HB-EGF activates two EGF receptor subtypes, HER1/ErbB1 and HER4 and binds to heparan sulfate proteoglycan.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: None
Constituents: <40% Trehalose, PBS, BSA
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Purification notes
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Purified.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HB EGF antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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Search PubMed (MEDLINE) for references to HB EGF
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HB EGF antibody - more information
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