Anti-HBEGF/DTR antibody (ab92620)
Key features and details
- Rabbit polyclonal to HBEGF/DTR
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HBEGF/DTR antibody
See all HBEGF/DTR primary antibodies -
Description
Rabbit polyclonal to HBEGF/DTR -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: ICC/IF -
Species reactivity
Reacts with: Human -
Immunogen
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab92620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 23 kDa). |
Target
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Function
May be involved in macrophage-mediated cellular proliferation. It is mitogenic for fibroblasts and smooth muscle but not endothelial cells. It is able to bind EGF receptors with higher affinity than EGF itself and is a far more potent mitogen for smooth muscle cells than EGF. Also acts as a diphtheria toxin receptor. -
Sequence similarities
Contains 1 EGF-like domain. -
Post-translational
modificationsSeveral N-termini have been identified by direct sequencing. The forms with N-termini 63, 73 and 74 have been tested and found to be biologically active.
O-linked glycan attachment sites were determined by Edman degradation, O-glycanase digest suggests mucin-type glycosylation (done in HB-EGF purified from histiocytic lymphoma cell line U-937). -
Cellular localization
Cell membrane and Secreted > extracellular space. Mature HB-EGF is released into the extracellular space and probably binds to a receptor. - Information by UniProt
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Database links
- Entrez Gene: 1839 Human
- Omim: 126150 Human
- SwissProt: Q99075 Human
- Unigene: 799 Human
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Alternative names
- diphtheria toxin receptor (heparin-binding EGF-like growth factor) antibody
- diphtheria toxin receptor (heparin-binding epidermal growth factor-like growth factor) antibody
- Diphtheria toxin receptor antibody
see all
Images
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Anti-HBEGF/DTR antibody (ab92620) at 1 µg/ml + Human stomach tumor tissue lysate (adenocarcinoma) - total protein (ab29678) at 10 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 23 kDa
Exposure time: 4 minutes
The band observed at 20 kDa could potentially be a cleaved form of HB EGF due to the presence of a 19 amino acid signal peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab92620 has been referenced in 8 publications.
- Yi L et al. CircRNA_30032 promotes renal fibrosis in UUO model mice via miRNA-96-5p/HBEGF/KRAS axis. Aging (Albany NY) 13:12780-12799 (2021). PubMed: 33973871
- Xiang J et al. Elevated HB-EGF expression in neural stem cells causes middle age obesity by suppressing Hypocretin/Orexin expression. FASEB J 35:e21345 (2021). PubMed: 33715219
- Tang H et al. Forkhead Box Q1 Is Critical to Angiogenesis and Macrophage Recruitment of Colorectal Cancer. Front Oncol 10:564298 (2020). PubMed: 33330033
- Xu K et al. Upregulated miR-96-5p inhibits cell proliferation by targeting HBEGF in T-cell acute lymphoblastic leukemia cell line. Folia Histochem Cytobiol 58:219-226 (2020). PubMed: 32893872
- Tornabene E et al. Effects of oxygen-glucose deprivation (OGD) on barrier properties and mRNA transcript levels of selected marker proteins in brain endothelial cells/astrocyte co-cultures. PLoS One 14:e0221103 (2019). PubMed: 31425564
- Zhang Y et al. Hypothalamic stem cells control ageing speed partly through exosomal miRNAs. Nature 548:52-57 (2017). PubMed: 28746310
- Gaviglio AL et al. Heparin-binding epidermal growth factor-like growth factor promotes neuroblastoma differentiation. FASEB J 31:1903-1915 (2017). PubMed: 28174207
- Altonsy MO et al. Context-dependent cooperation between nuclear factor ?B (NF-?B) and the glucocorticoid receptor at a TNFAIP3 intronic enhancer: a mechanism to maintain negative feedback control of inflammation. J Biol Chem 289:8231-9 (2014). WB ; Human . PubMed: 24500711