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Overview

  • Product nameAnti-HB9/HLXB9 antibodySee all HB9/HLXB9 primary antibodies ...
  • Description
    Sheep polyclonal to HB9/HLXB9
  • Specificityab59795 is specific for for HB9/HLXB9.
  • Tested applicationsIHC-P, WB, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide: H-APADRLRAESPSPPRLLAAHC-NH2, corresponding to amino acids 68-88 of Human HB9/HLXB9 conjugated to Blue Carrier Protein.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlNormal Term and First Trimester human Placenta and mouse Neuroblastoma and Spinal Cord cells.
  • General notesFor additional stability glycerol at a concentration of 1:1 can be added.

Properties

Applications

Our Abpromise guarantee covers the use of ab59795 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: Use at an assay dependent concentration.
WB WB: 1/1000. Predicted molecular weight: 41 kDa.
ELISA ELISA: 1/500 - 1/6500.

Target

  • FunctionPutative transcription factor involved in pancreas development and function.
  • Tissue specificityExpressed in lymphoid and pancreatic tissues.
  • Involvement in diseaseDefects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
  • Sequence similaritiesContains 1 homeobox DNA-binding domain.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession P50219 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        HB 9 antibodyHB9 antibodyHLXB 9 antibody
        HLXB9 antibodyHomeo box HB9 antibodyHomeobox HB9 antibodyHomeobox protein HB9 antibodyHOXHB9 antibodyMNX1 antibodyMNX1_HUMAN antibodyMotor neuron and pancreas homeobox protein 1 antibodySacral agenesis autosomal dominant (Currarino triad) antibodySCRA 1 antibodySCRA1 antibody
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    References for Anti-HB9/HLXB9 antibody (ab59795)

    This product has been referenced in:
    • Zoldan J  et al. The influence of scaffold elasticity on germ layer specification of human embryonic stem cells. Biomaterials 32:9612-21 (2011). IHC-P ; Human . Read more (PubMed: 21963156) »
    • Neufing PJ  et al. Expression and localization of homeodomain proteins DLX4/HB9 in normal and malignant human breast tissues. Anticancer Res 23:1479-88 (2003). IHC-P ; Human . Read more (PubMed: 12820413) »

    See all 2 Publications for this product

    Publishing research using ab59795 ? Please let us know so that we can cite the reference in this datasheet

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    Submit an Abreview for ab59795 Submit a Question for ab59795

    Displaying 1 - 5 of 5 results for Abreviews and Q&A

    10
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    My antigen retrieval was done by boiling in 10 mM sodium citrate for 20 min. I did not know whether secondary Ab is the issue (will do). The dilution of the antibody was 1:100 and incubated for 2 hr @ RT. See pic ab59795

    I have found a paper describing development of the HB9 antibody ab59795 and the protocol that was used for IHC on tumor tissue.

    Neufing PJ et al. Expression and localization of homeodomain proteins DLX4/HB9 in normal and malignant human breast...

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    We are sure this HB9 is present in the prostate tissue and prostate cell lines with a level of higher than normally seen proteins– we have done mRNA and western blotting in these tissue and cell lines. Normally we load 20 ug for WB for most of the p...

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    Thank you for the reassurance. If the IHC continues to be a problem, please contact me. Good luck!

    Thank you for the detailed reply! The other antibody I used is ab79541. I haven’t rule out the secondary yet (which was from Vector labs biotynilation – sheep IgG). Will do this later and also try other options as 1) blocking endogenous biot...

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    I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Abpro...

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    My antigen retrieval was done by boiling in 10 mM sodium citrate for 20 min. I did not know whether secondary Ab is the issue (will do). The dilution of the antibody was 1:100 and incubated for 2 hr @ RT. See pic ab59795 In addition, I tested another Abc...

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    Thank you for sending the details of your protocol. I agree that the staining you see for both of these antibodies is not what is expected. I think what you want is shown on the datasheet for a different antibody against HB9, ab92606, showing a stain of m...

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    We recently purchased the sheep polyclonal anti-HB9 antibody (ab59795) for IHC staining of human prostate tissue. Following standard IHC procedure, our IHC staining background turned out to be very high using anti-sheep IgG from Vector lab (tissue was blo...

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    Thank you for bringing this to our attention. Can you please send a few more details of your protocol? In particular, I would like to know if you included an antigen retrieval step. Also, did you confirm that the secondary is not the issue, by leaving out...

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