Anti-HB9/HLXB9 antibody (ab94360)

Overview

• Product nameAnti-HB9/HLXB9 antibodySee all HB9/HLXB9 primary antibodies ...
• Description
  Rabbit polyclonal to HB9/HLXB9
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human HB9/HLXB9.

  Read Abcam's proprietary immunogen policy

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: PANC-1; U937; K562; MOLT4.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Developmental Biology
  • Lineage specification
  • Ectoderm

  • Stem Cells
  • Neural Stem Cells
  • Neuron Restricted Lineage

  • Stem Cells
  • Lineage Markers
  • Ectoderm

  • Homeodomain

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • Developmental Families
  • Other

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Soma marker

Applications

Target

• FunctionPutative transcription factor involved in pancreas development and function.
• Tissue specificityExpressed in lymphoid and pancreatic tissues.
• Involvement in diseaseDefects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
• Sequence similaritiesContains 1 homeobox DNA-binding domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P50219 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3110 Human
  • Omim: 142994 Human
  • SwissProt: P50219 Human
  • Unigene: 37035 Human

• Alternative names
  HB 9 antibodyHB9 antibodyHLXB 9 antibody

  HLXB9 antibodyHomeo box HB9 antibodyHomeobox HB9 antibodyHomeobox protein HB9 antibodyHOXHB9 antibodyMNX1 antibodyMNX1_HUMAN antibodyMotor neuron and pancreas homeobox protein 1 antibodySacral agenesis autosomal dominant (Currarino triad) antibodySCRA 1 antibodySCRA1 antibody

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Anti-HB9/HLXB9 antibody images

• 

  Western blot - HB9/HLXB9 antibody (ab94360)
  All lanes : Anti-HB9/HLXB9 antibody (ab94360) at 1 µg/ml
  
  Lane 1 : PANC-1 (Human pancreatic carcinoma, epithelial-like cell line) Nuclear Lysate (ab14882)
  Lane 2 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate
  Lane 3 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
  Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 41 kDa
  Observed band size : 44 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 48 kDa,85 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 12 minutes