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RabMAbs (2570-1)

Anti-HB9/HLXB9 antibody [EPR3342] (ab79541)

Submit an Abreview Q&A (1)Specific References (2)

Overview

  • Product nameAnti-HB9/HLXB9 antibody [EPR3342]See all HB9/HLXB9 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3342] to HB9/HLXB9
  • Tested applicationsWB, IP, IHC-P more details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

    Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminal of Human HB9/HLXB9.

  • Positive controlMolt-4 and Raji cell lysates.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab79541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IP IP: 1/50.
IHC-P IHC-P: Use at an assay dependent concentration. PubMed: 21484430
  • Application notesIs unsuitable for ,Flow Cyt or ICC.

    • Target

    • FunctionPutative transcription factor involved in pancreas development and function.
    • Tissue specificityExpressed in lymphoid and pancreatic tissues.
    • Involvement in diseaseDefects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
    • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession P50219 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      • Alternative names
          HB 9 antibodyHB9 antibodyHLXB 9 antibody
          HLXB9 antibodyHomeo box HB9 antibodyHomeobox HB9 antibodyHomeobox protein HB9 antibodyHOXHB9 antibodyMNX1 antibodyMNX1_HUMAN antibodyMotor neuron and pancreas homeobox protein 1 antibodySacral agenesis autosomal dominant (Currarino triad) antibodySCRA 1 antibodySCRA1 antibody
        see all

      Anti-HB9/HLXB9 antibody [EPR3342] images

      • Western blot - HB9/HLXB9 antibody [EPR3342] (ab79541)
        Western blot - HB9/HLXB9 antibody [EPR3342] (ab79541)
        All lanes : Anti-HB9/HLXB9 antibody [EPR3342] (ab79541) at 1/200000 dilution

        Lane 1 : Molt-4 cell lysate
        Lane 2 : Raji cell lysate

        Lysates/proteins at 10 µg per lane.

        Secondary
        HRP labelled goat anti-rabbit at 1/2000 dilution

        Predicted band size : 41 kDa
        Observed band size : 48 kDa (why is the actual band size different from the predicted?)

      References for Anti-HB9/HLXB9 antibody [EPR3342] (ab79541)

      This product has been referenced in:
      • Egawa N  et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . Read more (PubMed: 22855461) »
      • Wilkens L  et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). WB, IHC-P ; Human . Read more (PubMed: 21484430) »

      See all 2 Publications for this product

      Publishing research using ab79541 ? Please let us know so that we can cite the reference in this datasheet

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      Votes: Highest First

      Thank you for the detailed reply! The other antibody I used is ab79541. I haven’t rule out the secondary yet (which was from Vector labs biotynilation – sheep IgG). Will do this later and also try other options as 1) blocking endogenous biot...

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      I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Abpro...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"