Overview

  • Product nameAnti-HB9/HLXB9 antibody [EPR3342]See all HB9/HLXB9 primary antibodies ...
  • Description
    Rabbit monoclonal [EPR3342] to HB9/HLXB9
  • Tested applicationsWB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Human

    Does not react with

    Rat
  • Immunogen

    A synthetic peptide corresponding to residues near the C terminal of Human HB9/HLXB9.

  • Positive control
    • Molt-4 and Raji cell lysates.
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab79541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 48 kDa (predicted molecular weight: 41 kDa).
IP 1/50.
IHC-P Use at an assay dependent concentration. PubMed: 21484430
  • Application notesIs unsuitable for Flow Cyt or ICC.
  • Target

    • FunctionPutative transcription factor involved in pancreas development and function.
    • Tissue specificityExpressed in lymphoid and pancreatic tissues.
    • Involvement in diseaseDefects in MNX1 are a cause of Currarino syndrome (CURRAS) [MIM:176450]. The triad of a presacral tumor, sacral agenesis and anorectal malformation constitutes the Currarino syndrome which is caused by dorsal-ventral patterning defects during embryonic development. The syndrome occurs in the majority of patients as an autosomal dominant trait.
    • Sequence similaritiesContains 1 homeobox DNA-binding domain.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HB 9 antibody
      • HB9 antibody
      • HLXB 9 antibody
      • HLXB9 antibody
      • Homeo box HB9 antibody
      • Homeobox HB9 antibody
      • Homeobox protein HB9 antibody
      • HOXHB9 antibody
      • MNX1 antibody
      • MNX1_HUMAN antibody
      • Motor neuron and pancreas homeobox protein 1 antibody
      • Sacral agenesis autosomal dominant (Currarino triad) antibody
      • SCRA 1 antibody
      • SCRA1 antibody
      see all

    Anti-HB9/HLXB9 antibody [EPR3342] images

    • All lanes : Anti-HB9/HLXB9 antibody [EPR3342] (ab79541) at 1/200000 dilution

      Lane 1 : Molt-4 cell lysate
      Lane 2 : Raji cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 41 kDa
      Observed band size : 48 kDa (why is the actual band size different from the predicted?)

    References for Anti-HB9/HLXB9 antibody [EPR3342] (ab79541)

    This product has been referenced in:
    • Egawa N  et al. Drug screening for ALS using patient-specific induced pluripotent stem cells. Sci Transl Med 4:145ra104 (2012). Human . Read more (PubMed: 22855461) »
    • Wilkens L  et al. The homeobox gene HLXB9 is upregulated in a morphological subset of poorly differentiated hepatocellular carcinoma. Virchows Arch 458:697-708 (2011). WB, IHC-P ; Human . Read more (PubMed: 21484430) »

    See all 2 Publications for this product

    Product Wall

    I will send the IHC protocol for ab59795 when I receive it, hoepfully in the next day or two. As I mentioned, Abcam does not validate this antibody for IHC in-house. We do not have free samples of any of our antibodies, but ab59795 is covered by our Ab...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"