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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
Overview
Product name
Anti-HBP1 antibody
See all HBP1 products (3) ...
Description
Rabbit polyclonal to HBP1
Tested applications
WB, ELISAmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Cow, Dog, Zebrafish
Immunogen
Synthetic peptide corresponding to a region within internal sequence amino acids 465-514 (SVILGDRWKK MKNEERRMYT LEAKALAEEQ KRLNPDCWKR KRTNSGSQQH) of Human HBP1 (NP_036389).
SVILGDRWKK MKNEERRMYT LEAKALAEEQ KRLNPDCWKR KRTNSGSQQH
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 2% Sucrose, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Stem Cells >> Signaling Pathways >> Wnt >> Nuclear
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HMG Box
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Western blot - HBP1 antibody (ab83402)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab83402 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
- 1 Image
WB
ELISA
Application notes
ELISA: Use at an assay dependent dilution. ELISA titre using peptide based assay: 1/1562500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 58 kDa (predicted molecular weight: 58 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Transcriptional repressor that binds to the promoter region of target genes. Plays a role in the regulation of the cell cycle and of the Wnt pathway. Binds preferentially to the sequence 5'-TTCATTCATTCA-3'. Binding to the H1F0 promoter is enhanced by interaction with RB1. Disrupts the interaction between DNA and TCF4.
Sequence similarities
Contains 1 AXH domain.
Contains 1 HMG box DNA-binding domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO60381
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- FLJ16340 antibody
- HBP 1 antibody
- HBP1 antibody
see all
Database links
- Entrez Gene: 515320 Cow
- Entrez Gene: 26959 Human
- Entrez Gene: 73389 Mouse
- Entrez Gene: 27080 Rat
- SwissProt: Q2KJ34 Cow
- SwissProt: O60381 Human
- SwissProt: Q8R316 Mouse
- SwissProt: Q62661 Rat
see all
Anti-HBP1 antibody images:
Western blot - HBP1 antibody (ab83402)
Anti-HBP1 antibody (ab83402) at 1 µg/ml (in 5% skim milk / PBS buffer) + HeLa cell lysate at 10 µg
Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size : 58 kDa
Observed band size : 58 kDa
Gel concentration: 12%
References for Anti-HBP1 antibody (ab83402)
This product has been referenced in:
- Wang Wet al. The acetylation of transcription factor HBP1 by p300/CBP enhances p16INK4A expression. Nucleic Acids Res : (2011). WB; Human.Read more (PubMed: 21967847) »
- Li Het al. miR-17-5p promotes human breast cancer cell migration and invasion through suppression of HBP1. Breast Cancer Res Treat 126:565-75 (2011). WB; Human.Read more (PubMed: 20505989) »
See all 2 publications for this product
Publishing research using ab83402? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"