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Overview

  • Product nameHBsAg adr protein
  • Protein descriptionRecombinant Full Length HBsAg (Hepatitis B Surface Antigen), adr subtype, produced in Saccharomyces cerevisae. Contains 227 amino acids, M.wt 24 kDa. (Small amounts of dimer and trimer forms also exist in the solution.)
  • Expression hostSaccharomyces cerevisiae

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity:Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE
  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Sodium azide
    Constituents: PBS, 0.8% Sodium chloride
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab73749 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Major surface antigenHBV major surface antigenHBV surface antigenS
  • RelevanceHBsAg is the surface antigen of the Hepatitis-B-Virus (HBV). The antigenic determinant of the protein moiety of the HBsAg determines specific characteristics of different serotypes and provides the basis of immunodetection. HBsAg has antigenic heterogeneity, specifically, two pairs of sub specific determinants, d/y and w/r allow the following combinations: adw, ayw, adr, ayr.

    • References for HBsAg adr protein (ab73749)

    This product has been referenced in:
    • Hempel F  et al. Algae as Protein Factories: Expression of a Human Antibody and the Respective Antigen in the Diatom Phaeodactylum tricornutum. PLoS One 6:e28424 (2011). Read more (PubMed: 22164289) »

    See 1 Publication for this product

    Publishing research using ab73749 ? Please let us know so that we can cite the reference in this datasheet

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    I can understand that the information regarding the exact sequence is proprietary. Would you however be able to tell us the genotype (A-F) of both antigens used?

    Kind regards

    Thank you for your reply.

    All of the information that we have about ab91276 is that amino acids 1-226, 25kDa of UniProt ID = Q773S4 was used to generate the protein. For ab73749, I do not have anymore information other than what is already on th...

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    We recently acquired two of your HBsAg (ab73749 & 91276).

    You do provide online information regarding the vector used for production and which strain it is, but since there are even differences between countries (A to F) and within one anti...

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    Thank you for contacting Abcam.

    For ab91276, amino acids 1-226, 25kDa of UniProt ID = Q773S4 was used to generate the protein, but for ab73749 the information is considered is proprietary.

    If there is anything else I can help you with,...

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    Hi
    I am interested in purchasing HBsAg adr protein (ab73749).
    However I want to choose the most common sub specific determinant for the
    Sub Saharan region.
    Would you advise adw, adr, ayr or ayw?
    A speedy response would be apprecia...

    Read More

    Thank you for contacting us.

    We are happy to help customers find the most suitable products for their research purposes; however we are not specialized in this particular field of virology and do not want to recommend a product that may be unsu...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"