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Products:Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Overview
Product name
Anti-HCCS antibody
See all HCCS products (2) ...
Description
Rabbit polyclonal to HCCS
Tested applications
ELISA, WB, IHC-P, ICC/IFmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide derived from an internal sequence of human HCCS.
Positive control
Human tonsil tissue, HeLa, HepG2 and COLO cell extracts. MCF7 cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Metabolism >> Mitochondrial
Cardiovascular >> Lipids / Lipoproteins >> Lipid Metabolism >> Cytochromes
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74801 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/20000ELISA: 1/20000
WB: 1/500 - 1/1000.Predicted molecular weight: 31 kDa.
IHC-P: 1/50 - 1/100.
ICC/IF: 1/500 - 1/1000.
Target
Relevance
HCCS links covalently the heme group to the apoprotein of cytochrome c.
Cellular localization
Mitochondrial inner membrane
Alternative names
- OTTHUMP00000022904 antibody
- OTTHUMP00000022905 antibody
- CCHL antibody
see all
Database links
- Entrez Gene: 3052 Human
- Entrez Gene: 15159 Mouse
- Omim: 300056 Human
- SwissProt: P53701 Human
- SwissProt: P53702 Mouse
Anti-HCCS antibody images:
Western blot - HCCS antibody (ab74801)
All lanes : Anti-HCCS antibody (ab74801) at 1/500 dilution
Lane 1 : HeLa cell extracts
Lane 2 : HepG2 cell extracts
Lane 3 : COLO cell extracts
Lane 4 : HeLa cell extracts with immunising peptide at 10 µg
Lysates/proteins at 30 µg/ml per lane.
Predicted band size : 31 kDa
Observed band size : 31 kDa
Additional bands at : 84 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - HCCS antibody (ab74801)
ab74801 at 1/500 dilution staining HCCS in MCF7 by Immunofluorescence, in the absence or presence of the immunising peptide.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HCCS antibody (ab74801)
ab74801 at 1/50 dilution staining HCCS in human tonsil by Immunohistochemistry, Paraffin-embedded tissue, in the absence or presence of the immunising peptide.
References for Anti-HCCS antibody (ab74801)
ab74801 has not yet been referenced specifically in any publications.
Publishing research using ab74801? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"