Anti-HCN1 antibody - C-terminal (ab229340)
Key features and details
- Rabbit polyclonal to HCN1 - C-terminal
- Suitable for: IHC-Fr, ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-HCN1 antibody - C-terminal
See all HCN1 primary antibodies -
Description
Rabbit polyclonal to HCN1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment within Human HCN1 (C terminal). The exact sequence is proprietary.
Database link: O60741 -
Positive control
- WB: Mouse and rat brain tissue extracts; SK-N-AS whole cell extract. IHC-P: Mouse retina and brain tissues; rat brain tissue. ICC/IF: Rat DIV9 E18 primary cortical neurons. IHC-Fr: Frozen mouse cerebellum and hippocampus tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
1/100 - 1/1000.
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ICC/IF |
1/100 - 1/1000.
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 99 kDa.
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Notes |
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IHC-Fr
1/100 - 1/1000. |
ICC/IF
1/100 - 1/1000. |
IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 99 kDa. |
Target
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Function
Hyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli. -
Tissue specificity
Detected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas. -
Sequence similarities
Belongs to the potassium channel HCN family.
Contains 1 cyclic nucleotide-binding domain. -
Domain
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position. -
Cellular localization
Membrane. - Information by UniProt
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Database links
- Entrez Gene: 348980 Human
- Entrez Gene: 15165 Mouse
- Entrez Gene: 84390 Rat
- Omim: 602780 Human
- SwissProt: O60741 Human
- SwissProt: O88704 Mouse
- SwissProt: Q9JKB0 Rat
- Unigene: 650434 Human
see all -
Alternative names
- BCNG-1 antibody
- BCNG1 antibody
- Brain cyclic nucleotide gated channel 1 antibody
see all
Images
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Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution + SK-N-AS (human neuroblastoma cell line) whole cell extract at 30 µg
Predicted band size: 99 kDa7.5% SDS-PAGE gel.
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4% paraformaldehyde-fixed DIV9 rat E18 primary cortical neurons cells stained for HCN1 (green) using ab229340 at 1/500 dilution in ICC/IF.
Beta Tubulin 3/ Tuj1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Paraffin-embedded rat brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.
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Paraffin-embedded mouse brain tissue stained for HCN1 using ab229340 at 1/500 dilution in immunohistochemical analysis.
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Frozen-sectioned adult mouse hippocampus tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.
Nuclear counterstain: Fluoroshield with DAPI (blue)
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All lanes : Anti-HCN1 antibody - C-terminal (ab229340) at 1/1000 dilution
Lane 1 : Mouse brain tissue extract
Lane 2 : Rat brain tissue extract
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 99 kDa7.5% SDS-PAGE gel.
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Frozen-sectioned adult mouse cerebellum tissue stained for HCN1 (green) using ab229340 at 1/250 dilution in immunohistochemical analysis.
beta Tubulin 3/ TUJ1 (red).
Nuclear counterstain: Fluoroshield with DAPI (blue).
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Paraffin-embedded mouse retina tissue stained for HCN1 using ab229340 at 1/800 dilution in immunohistochemical abalysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab229340 has been referenced in 3 publications.
- Zhong W & Darmani NA The HCN Channel Blocker ZD7288 Induces Emesis in the Least Shrew (Cryptotis parva). Front Pharmacol 12:647021 (2021). PubMed: 33995059
- Hughes BA et al. Chronic ethanol exposure alters prelimbic prefrontal cortical Fast-Spiking and Martinotti interneuron function with differential sex specificity in rat brain. Neuropharmacology 162:107805 (2020). PubMed: 31589884
- Kashyap M et al. Constitutively active HCN channels constrain detrusor excitability and modulate evoked contractions of human bladder. Am J Clin Exp Urol 8:163-176 (2020). PubMed: 33235894