Overview

  • Product nameAnti-HCN1 antibody [S70-28]
    See all HCN1 primary antibodies
  • Description
    Mouse monoclonal [S70-28] to HCN1
  • SpecificityNo cross reactivity against HCN2
  • Tested applicationsIHC-P, IHC-Fr, IHC-FoFr, IP, WB, ICC/IF, Flow Cytmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Fusion protein, corresponding to cytoplasmic C terminal amino acids 778-910 of Rat HCN1 (accession number Q9JKBo)

  • Positive control
    • Rat brain lysate

Properties

Applications

Our Abpromise guarantee covers the use of ab84816 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent dilution.
IHC-Fr Use at an assay dependent dilution.
IHC-FoFr Use at an assay dependent concentration. PubMed: 22948144
IP Use at an assay dependent dilution.
WB Use a concentration of 1 - 10 µg/ml. Detects a band of approximately 99 kDa (predicted molecular weight: 99 kDa).
ICC/IF Use a concentration of 1 - 10 µg/ml.
Flow Cyt Use 1-2µg for 106 cells.

Target

  • FunctionHyperpolarization-activated ion channel exhibiting weak selectivity for potassium over sodium ions. Contributes to the native pacemaker currents in heart (If) and in neurons (Ih). Activated by cAMP, and at 10-100 times higher concentrations, also by cGMP. May mediate responses to sour stimuli.
  • Tissue specificityDetected in brain, in particular in amygdala and hippocampus, while expression in caudate nucleus, corpus callosum, substantia nigra, subthalamic nucleus and thalamus is very low or not detectable. Detected at very low levels in muscle and pancreas.
  • Sequence similaritiesBelongs to the potassium channel HCN family.
    Contains 1 cyclic nucleotide-binding domain.
  • DomainThe segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.
  • Cellular localizationMembrane.
  • Information by UniProt
  • Database links
  • Alternative names
    • BCNG-1 antibody
    • BCNG1 antibody
    • Brain cyclic nucleotide gated channel 1 antibody
    • Brain cyclic nucleotide-gated channel 1 antibody
    • HAC 2 antibody
    • HAC2 antibody
    • HCN1 antibody
    • HCN1_HUMAN antibody
    • Hyperpolarization activated cyclic nucleotide gated potassium channel 1 antibody
    • Potassium channel, voltage-gated, brain, 1 antibody
    • Potassium/sodium hyperpolarization activated cyclic nucleotide gated channel 1 antibody
    • Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 1 antibody
    see all

Anti-HCN1 antibody [S70-28] images

  • ab84816 staining HCN1 in human hippocampal tissue by IHC-P (Bouin's fixed paraffin embedded).
  • Anti-HCN1 antibody [S70-28] (ab84816) + rat brain lysate

    Predicted band size : 99 kDa
    Observed band size : 99 kDa
  • Overlay histogram showing SH-SY5Y cells stained with ab84816 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab84816, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in SH-SY5Y cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween for 20 min used under the same conditions.

  • Immunohistochemical analysis of murine cochlear tissue, staining HCN1 (red) with ab84816.

    Tissue was permeabilized with 0.3% Triton X-100 before being blocked with goat serum. Sections were incubated with primary antibody (1/100) overnight at 4°C. An AlexaFluor®568-conjugated goat anti-mouse IgG (1/2000) was used as the secondary antibody.

References for Anti-HCN1 antibody [S70-28] (ab84816)

This product has been referenced in:
  • Nava C  et al. De novo mutations in HCN1 cause early infantile epileptic encephalopathy. Nat Genet 46:640-5 (2014). WB ; Human . Read more (PubMed: 24747641) »
  • Cao-Ehlker X  et al. Up-regulation of hyperpolarization-activated cyclic nucleotide-gated channel 3 (HCN3) by specific interaction with K+ channel tetramerization domain-containing protein 3 (KCTD3). J Biol Chem 288:7580-9 (2013). Read more (PubMed: 23382386) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (HL-1 cardiac cell line)
Specification HL-1 cardiac cell line
Fixative Methanol
Blocking step Goat serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Nov 15 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"