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Hepatitis C Virus NS3 protein (Active) (ab49025)

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Overview

  • Product nameHepatitis C Virus NS3 protein (Active)See all Hepatitis C Virus NS3 proteins and peptides ...
  • Protein descriptionRecombinant fusion protein, corresponding to amino acids 1450-1643 of HCV NS3 (22 kDa), beta-galactosidase (114 kDa) fused at the N-terminal.
  • Expression hostE. coli

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Purification notesPurity of proteins is evaluated by SDS-PAGE
  • Biological activityReactivity with human HCV positive serum undetermined.
  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 50% Glycerol, 8M Urea, 20mM Tris HCl, 10mM Beta mercaptoethanol, pH 8
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab49025 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    ELISA ELISA: Use at an assay dependent dilution.
    Flow Cyt Flow Cyt: Use at an assay dependent dilution.
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent dilution.

    Protein info

    • Alternative names
        Hepatitis C Virus NS3NS3NS3P
        p70Serine protease/NTPase/helicase
      see all
  • RelevanceHCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa.
  • Cellular localizationEndoplasmic reticulum membrane

    • References for Hepatitis C Virus NS3 protein (Active) (ab49025)

    ab49025 has not yet been referenced specifically in any publications.

    Publishing research using ab49025 ? Please let us know so that we can cite the reference in this datasheet

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    Dear Colleagues, My customer is interested in one of the products: ab49022, ab49024, ab49025. She would like to know for how many standard Flow Cyt applications they are dedicated? I would be grateful for your help and quick response. Best regards,...

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    Thank you for your enquiry and your interest in our products. Generally, one vial of 100 ug of protein of these products (ab49022, ab49024, ab49025) typically can give 50 to 100 tests depending on the format. More tests can be performed for ELISA a...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"