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Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263)

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Overview

Product name

Anti-HDAC1 antibody [1.T.9] - ChIP Grade
See all HDAC1 products (22) ...

Description

Mouse monoclonal [1.T.9] to HDAC1 - ChIP Grade

Specificity

ab31263 recognizes HDAC1 (Histone Deacetylase 1).

Tested applications

WB, ICC, IP, ChIP, ICC/IF, IHC-FoFrmore details

Cross reactivity

Reacts with

Mouse, Cow, Human

Predicted to work with

Rat, Dog, Chimpanzee

Immunogen

Synthetic peptide: CEEKPEAKGVKEEVKLA conjugated to KLH, corresponding to amino acids 467-482 of Mouse HDAC1

CEEKPEAKGVKEEVKLA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

3T3/A31 cell lysate

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 30% Glycerol, 0.15M Sodium chloride, 0.1M Tris glycine. pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

1.T.9

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

Applications

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Our Abpromise guarantee covers the use of ab31263 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp686H12203 antibody
  • GON 10 antibody
  • HD 1 antibody
  • HD1 antibody
  • HD1 antibody
  • HDAC 1 antibody
  • HDAC1 antibody
  • hdac1: histone deacetylase 1 antibody
  • hdac1: histone deacetylase 1 antibody
  • HDAC1_HUMAN antibody
  • Histone deacetylase 1 (HD1) antibody
  • Histone deacetylase 1 (HD1) antibody
  • Histone deacetylase 1 antibody
  • Reduced potassium dependency yeast homolog like 1 antibody
  • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
  • RPD3 (reduced potassium dependency) antibody
  • RPD3 (reduced potassium dependency) antibody
  • RPD3 antibody
  • RPD3 antibody
  • RPD3L1 antibody
  • RPD3L1 antibody
see all

Database links

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References for Anti-HDAC1 antibody [1.T.9] - ChIP Grade (ab31263)

This product has been referenced in:

  • Martinez FP & Tang Q The leucine zipper domain is required for the Kaposi Sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem : (2012). WB, ICC/IF; Human.Read more (PubMed: 22416134) »
  • Varlakhanova N  et al. Myc and Miz-1 have coordinate genomic functions including targeting Hox genes in human embryonic stem cells. Epigenetics Chromatin 4:20 (2011). ChIP; Human.Read more (PubMed: 22053792) »

See all 6 publications for this product

Publishing research using ab31263? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"