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Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985)

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Overview

Product name

Anti-HDAC1 antibody [10E2] - ChIP Grade
See all HDAC1 products (22) ...

Description

Mouse monoclonal [10E2] to HDAC1 - ChIP Grade

Specificity

This antibody recognizes HDAC1.

Tested applications

WB, ICC/IF, IP, ChIPmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide: EEKPEAKGVKEEVKLA, corresponding to C terminal amino acids 467-482 of Human and mouse HDAC1

EEKPEAKGVKEEVKLA

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

HeLa cells.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.035% Sodium Azide
Constituents: 30% Glycerol

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

10E2

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - HDAC1 antibody [10E2] - ChIP Grade (ab46985)Western blot - HDAC1 antibody [10E2] - ChIP Grade (ab46985) image (enlarge)

  • ChIP - Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985)ChIP - Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab46985 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide2 Images

    WB

    Four stars (1 Abreview) WB: 1/500 - 1/2000. Dete...Read more →

    WB: 1/500 - 1/2000. Detects a band of approximately 57 kDa (predicted molecular weight: 55 kDa).

  • ICC/IF

     ICC/IF: 1/50 - 1/200.

    ICC/IF: 1/50 - 1/200.

  • ShowHide

    IP

     IP: Use at an assay depe...Read more →

    IP: Use at an assay dependent dilution.

  • ShowHide1 Image

    ChIP

    Five stars (1 Abreview) ChIP: 1/10 - 1/50. This ...Read more →

    ChIP: 1/10 - 1/50. This antibody was validated for ChIP using 10 ul of antibody added to chromatin prepared from formaldehyde fixed U2OS cells. The p21 gene promoter was interrogated by PCR. The promoter of the HDAC1 gene was interrogated for the presence of HDAC1 from chromatin harvested from fixed Swiss 3T3 cells in separate experiements.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp686H12203 antibody
  • GON 10 antibody
  • HD 1 antibody
  • HD1 antibody
  • HDAC 1 antibody
  • HDAC1 antibody
  • hdac1: histone deacetylase 1 antibody
  • HDAC1_HUMAN antibody
  • Histone deacetylase 1 (HD1) antibody
  • Histone deacetylase 1 antibody
  • Reduced potassium dependency yeast homolog like 1 antibody
  • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
  • RPD3 (reduced potassium dependency) antibody
  • RPD3 antibody
  • RPD3L1 antibody
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Database links

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Anti-HDAC1 antibody [10E2] - ChIP Grade images:

  Western blot - HDAC1 antibody [10E2] - ChIP Grade (ab46985)

Western blot - HDAC1 antibody [10E2] - ChIP Grade (ab46985)

Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985) at 1/1000 dilution + Nuclear extract of HeLa cells.

Predicted band size : 55 kDa
Observed band size : 57 kDa (why is the actual band size different from the predicted?)

  ChIP - Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985)

ChIP - Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985)

ChIP analysis using ab46985 binding HDAC1 in Schwann cells. Cells were fixed with paraformaldehyde for 10 mins. Samples were incubated with primary antibody for 12 hours at 4°C at an antibody concentration of 0.1µg/µl of chromatin. Protein binding was detected using semi-quantitative PCR at both a positive and negative control regions.

Image courtesy of Tom Hu by Abreview.

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References for Anti-HDAC1 antibody [10E2] - ChIP Grade (ab46985)

This product has been referenced in:

  • Bantscheff M  et al. Chemoproteomics profiling of HDAC inhibitors reveals selective targeting of HDAC complexes. Nat Biotechnol : (2011).Read more (PubMed: 21258344) »
  • Opsahl JA  et al. Increased interaction between DJ-1 and the Mi-2/ nucleosome remodelling and deacetylase complex during cellular stress. Proteomics 10:1494-504 (2010). ICC/IF; Human.Read more (PubMed: 20127688) »

See all 6 publications for this product

Publishing research using ab46985? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"