Anti-HDAC1 antibody (ab7028)
Key features and details
- Rabbit polyclonal to HDAC1
- Suitable for: ICC/IF, WB, IP
- Reacts with: Mouse, Rat, Human, Chinese hamster
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-HDAC1 antibody
See all HDAC1 primary antibodies -
Description
Rabbit polyclonal to HDAC1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, Chinese hamster -
Immunogen
Synthetic peptide corresponding to Human HDAC1 aa 466-482 conjugated to Keyhole Limpet Haemocyanin (KLH).
Sequence:C-KEEKPEAKGVKEEVKLA
Database link: HDAC1 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
IgG fraction -
Purification notes
Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Assay kits
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab7028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (4) |
1/200.
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WB | (8) |
1/2000 - 1/20000. Predicted molecular weight: 55 kDa.
|
IP | (1) |
Use at an assay dependent concentration.
Use 5 -10 μL antibody per reaction |
Notes |
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ICC/IF
1/200. |
WB
1/2000 - 1/20000. Predicted molecular weight: 55 kDa. |
IP
Use at an assay dependent concentration. Use 5 -10 μL antibody per reaction |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
see all -
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
Images
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All lanes : Anti-HDAC1 antibody (ab7028) at 1/10000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate
Lane 3 : K562 (human chronic myelogenous leukemia cell line from bone marrow ) cell lysate
Lane 4 : CHO (Chinese hamster ovary cell line) cell lysate
Lane 5 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 6 : PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate
Secondary
All lanes : Goat Anti-Rabbit HRP
Predicted band size: 55 kDa -
MCF-7 cells were treated with indicated concentrations of panobinostat (optical sections A-D, respectively) or trichostatin A (optical sections E-H, respectively) for 12 hours, fixed, permeabilized and optical sections were obtained by laser scanning confocal microscopy. Fluorescence signal for HDAC1 is shown in green (left panels), DAPI staining is shown in blue (middle panels), and merged optical sections are shown in the right panels. Colocalization analysis of HDAC1 fluorescence signal and the DAPI stain signal was performed with JACoP (ImageJ).
HDAC1 was detected with ab7028. cells were ficed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
(After Figure 3 of Hanigan et al).
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HDAC1 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with ab7028.
Lane 1: 5 μl ab7028 in HeLa whole cell lysate.
Lane 2: 10 μl ab7028 in HeLa whole cell lysate.
Lane 3: 10 μl control antibody in HeLa whole cell lysate.
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Immunofluorescence analysis of methanol fixed HeLa cells with ab7028 labelling HDAC1 at 1/200 dilution. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.
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All lanes : Anti-HDAC1 antibody (ab7028) at 1/1000 dilution
Lane 1 : Whole cell lysate from human HEK293 cell line
Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 10 seconds
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HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 60ka: HDAC1. -
Anti-HDAC1 antibody (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg
Secondary
HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 55 kDa
Exposure time: 20 seconds
Blocked with 5% milk for 1 hour at 20°C
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (233)
ab7028 has been referenced in 233 publications.
- LeBlanc L et al. β-catenin links cell seeding density to global gene expression during mouse embryonic stem cell differentiation. iScience 25:103541 (2022). PubMed: 34977504
- Lex RK et al. GLI transcriptional repression is inert prior to Hedgehog pathway activation. Nat Commun 13:808 (2022). PubMed: 35145123
- Luo H et al. HOTTIP-dependent R-loop formation regulates CTCF boundary activity and TAD integrity in leukemia. Mol Cell 82:833-851.e11 (2022). PubMed: 35180428
- Bray D et al. CASCADE: high-throughput characterization of regulatory complex binding altered by non-coding variants. Cell Genom 2:N/A (2022). PubMed: 35252945
- Rivera C et al. Unveiling RCOR1 as a rheostat at transcriptionally permissive chromatin. Nat Commun 13:1550 (2022). PubMed: 35322029