Overview

  • Product nameAnti-HDAC1 antibody - ChIP GradeSee all HDAC1 primary antibodies ...
  • Description
    Rabbit polyclonal to HDAC1 - ChIP Grade
  • SpecificityAnti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kD).
  • Tested applicationsFlow Cyt, WB, IP, IHC-P, ChIP/Chip, ChIP, ICC/IF, IHC-Fr more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: C-KEEKPEAKGVKEEVKLA conjugated to KLH, corresponding to amino acids 466-482 of Human HDAC 1.

  • Positive control
    • HeLa human epithelioid carcinoma cells. Whole cell lysate from human HEK293 cell line.

Properties

Applications

Our Abpromise guarantee covers the use of ab7028 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Use at an assay dependent concentration.
WB 1/2000 - 1/20000. Predicted molecular weight: 55 kDa.
IP Use at an assay dependent concentration.
IHC-P 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol.
ChIP/Chip Use at an assay dependent concentration.
ChIP Use 5µl for 106 cells.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 23911933

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q13547 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD 1 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • hdac1: histone deacetylase 1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 (HD1) antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody - ChIP Grade images

  • HeLa cells were fixed and permeabilized with cold methanol followed by cold methanol:acetone. Fixed cells were stained with 1:200 ab7028. The antibody was developed using Goat Anti-Rabbit IgG, Cy3 conjugate.

  •  Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab7028 to HDAC1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5 µl of ab7028 and 2x10.

  • ab7028 staining HDAC1 in human primary cardiac cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized with permeabilization buffer. The sample was incubated with the undiluted primary antibody for 12 hours at 4°C. An Allophycocyanin-conjugated goat polyclonal anti-rabbit IgG (1/1000) was used as the secondary antibody.
    Gating Strategy: Isotype negative control (white).

    See Abreview

  • All lanes : Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/1000 dilution

    Lane 1 : Whole cell lysate from human HEK293 cell line
    Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA

    Lysates/proteins at 20 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa


    Exposure time : 10 seconds

    This image is courtesy of an anonymous Abreview

    See Abreview

  • Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg

    Secondary
    HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 55 kDa


    Exposure time : 20 seconds

    This image is courtesy of an anonymous Abreview

    Blocked with 5% milk for 1 hour at 20°C

    See Abreview

  • HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 60ka: HDAC1.

References for Anti-HDAC1 antibody - ChIP Grade (ab7028)

This product has been referenced in:
  • Singh AP & Archer TK Analysis of the SWI/SNF chromatin-remodeling complex during early heart development and BAF250a repression cardiac gene transcription during P19 cell differentiation. Nucleic Acids Res 42:2958-75 (2014). IP ; Mouse . Read more (PubMed: 24335282) »
  • Toska E  et al. Prohibitin is required for transcriptional repression by the WT1-BASP1 complex. Oncogene N/A:N/A (2013). Read more (PubMed: 24166496) »

See all 45 Publications for this product

Product Wall

Application Flow Cytometry
Fixation Paraformaldehyde
Permeabilization Yes - eBioscience Permeabilization Buffer
Sample Human Cell (Primary Cardiac Cells)
Specification Primary Cardiac Cells
Gating Strategy Isotype negative control (white)
Preparation Cell harvesting/tissue preparation method: Typsin-EDTA Cell Dissociation
Sample buffer: PBS and 10% FBS
Username

Abcam user community

Verified customer

Submitted Feb 20 2014

The antibody concentrations are:
Anti-HDAC1 antibody - ChIP Grade (ab7028), lot GR9959 - 0.2 mg/ml
Anti-BAF53A antibody (ab84486), lot GR41407 - 10.0 mg/ml.

Application Western blot
Sample Mouse Tissue lysate - whole (Forebrain Tissue)
Loading amount 200 µg
Specification Forebrain Tissue
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Nov 07 2012

Thank you for your reply.


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

To check the status of the order pl...

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Thank you for contacting Abcam regarding ab7028.


I am sorry that you have been experiencing difficutlies with this lot of antibody in ChIP. We do have another lot available that I will be happy to send as a replacement. Can you please pro...

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Application Western blot
Sample Human Cell lysate - whole cell (huh-7 cell line)
Loading amount 15 µg
Specification huh-7 cell line
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

Submitted May 04 2012

Thank you so much for keeping me updated about the situation, and I am so happy to hear that the replacement from a new lot is working well. Please let me know if there is anything else that you need, and have a great week!

Thanks for your call today and letting us know about the trouble with the new lot of ab7028. I am sending a free of charge vial from a new lot on the order ***, which should arrive tomorrow. Please keep me updated with the results using...

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Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (Glioma cell line C6)
Specification Glioma cell line C6
Fixative Paraformaldehyde
Permeabilization Yes - 0,1% Triton X 100
Blocking step BSA as blocking agent for 20 minute(s) · Concentration: 0.5% · Temperature: RT°C
Username

Abcam user community

Verified customer

Submitted Aug 31 2011

Thank you for your patience while I have been in touch with the lab regarding the antigen retrieval method for ab7028. I have heard from the lab that they used 0.1% pronase for 5 - 10 minutes in order to expose the epitope. Please let me know if...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"