Anti-HDAC1 antibody - ChIP Grade (ab7028)
Overview
- Product nameAnti-HDAC1 antibody - ChIP GradeSee all HDAC1 primary antibodies ...
- DescriptionRabbit polyclonal to HDAC1 - ChIP Grade
- SpecificityAnti-Histone Deacetylase 1 specifically recognizes histone deacetylase 1 by immunoblotting and immunoprecipitation (65 kD).
- Tested applicationsWB, IP, IHC-P, ChIP/Chip, ChIP, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide: C-KEEKPEAKGVKEEVKLA conjugated to KLH, corresponding to amino acids 466-482 of Human HDAC 1.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.01M PBS pH7.4, 15mM Na azide
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Concentration information loading... - PurityIgG fraction
- Purification notesWhole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab7028 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/2000 - 1/20000. Predicted molecular weight: 55 kDa. |
| IP | IP: Use at an assay dependent concentration. |
| IHC-P | IHC-P: 1/500. Perform enzymatic antigen retrieval before commencing with IHC staining protocol. |
| ChIP/Chip | ChIP/Chip: Use at an assay dependent dilution. |
| ChIP | ChIP: Use 5µl for 106 cells. |
| ICC/IF | ICC/IF: Use at an assay dependent dilution. |
Target
- FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
- Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
- Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
- Post-translational
modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. - Cellular localizationNucleus.
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Database links
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
- Unigene: 202504 Mouse
- Unigene: 391033 Mouse
- Unigene: 1863 Rat
see all
Target information above from: UniProt accession
Q13547
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody
- HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody
see all
Anti-HDAC1 antibody - ChIP Grade images
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ChIP - Anti-HDAC1 antibody - ChIP Grade (ab7028)Sylvain DaujatSonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab7028 to HDAC1 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 5µ l of ab7028 and 2x106 cells were used in each ChIP experiment. -
Western blot - HDAC1 antibody - ChIP Grade (ab7028)All lanes : Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/1000 dilution
Lane 1 : Whole cell lysate from human HEK293 cell line
Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC1 gene silencing shRNA
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55 kDa
Exposure time : 10 secondsThis image is courtesy of an anonymous Abreview
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Immunoprecipitation - Anti-HDAC1 antibody - ChIP Grade (ab7028)HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab7028.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 60ka: HDAC1. -
Western blot - Anti-HDAC1 antibody - ChIP Grade (ab7028)Anti-HDAC1 antibody - ChIP Grade (ab7028) at 1/2000 dilution + Human HuH-7 whole cell lysate at 15 µg
Secondary
HRP conjugated Sheet anti-rabbit IgG polyclonal at 1/20000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55 kDa
Exposure time : 20 secondsThis image is courtesy of an anonymous Abreview
References for Anti-HDAC1 antibody - ChIP Grade (ab7028)
This product has been referenced in:
- Rajabi H et al. MUC1-C oncoprotein induces TCF7L2 transcription factor activation and promotes cyclin D1 expression in human breast cancer cells. J Biol Chem 287:10703-13 (2012). ChIP ; Human . Read more (PubMed: 22318732) »
- Ichi S et al. Role of Pax3 acetylation in the regulation of Hes1 and Neurog2. Mol Biol Cell 22:503-12 (2011). ChIP ; Mouse . Read more (PubMed: 21169561) »
