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Anti-HDAC1 antibody [EPR460(2)] (ab109411)

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Overview

Product name

Anti-HDAC1 antibody [EPR460(2)]
See all HDAC1 products (22) ...

Description

Rabbit monoclonal [EPR460(2)] to HDAC1

Tested applications

WB, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues near the C-terminus of Human HDAC1.

Positive control

Jurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR460(2)

Isotype

IgG1

Research areas

Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - HDAC1 antibody [EPR460(2)] (ab109411)Western blot - HDAC1 antibody [EPR460(2)] (ab109411) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

Is unsuitable for ,Flow Cyt or IP.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp686H12203 antibody
  • GON 10 antibody
  • HD1 antibody
  • HDAC 1 antibody
  • HDAC1 antibody
  • HDAC1_HUMAN antibody
  • Histone deacetylase 1 antibody
  • Reduced potassium dependency yeast homolog like 1 antibody
  • RPD3 antibody
  • RPD3L1 antibody
see all

Database links

see all

Anti-HDAC1 antibody [EPR460(2)] images:

  Western blot - HDAC1 antibody [EPR460(2)] (ab109411)

Western blot - HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.


Predicted band size : 55 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.

References for Anti-HDAC1 antibody [EPR460(2)] (ab109411)

ab109411 has not yet been referenced specifically in any publications.

Publishing research using ab109411? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"