Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Overview

• Product nameAnti-HDAC1 antibody [EPR460(2)]See all HDAC1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR460(2)] to HDAC1
• Tested applicationsICC/IF, WB, IHC-P, ICC more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C-terminus of Human HDAC1.

• Positive controlJurkat, HeLa, K562, and MCF7 cell lysates; Human colon tissue; Human tonsil tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR460(2)
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
• Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
• Post-translational
  modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
  Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
  Ubiquitinated by CHFR, leading to its degradation by the proteasome.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3065 Human
  • Entrez Gene: 433759 Mouse
  • Entrez Gene: 297893 Rat
  • Omim: 601241 Human
  • SwissProt: Q13547 Human
  • SwissProt: O09106 Mouse
  • SwissProt: Q4QQW4 Rat
  • Unigene: 88556 Human

  • Unigene: 202504 Mouse
  • Unigene: 391033 Mouse
  • Unigene: 1863 Rat

  see all

• Alternative names
  DKFZp686H12203 antibodyGON 10 antibodyHD1 antibody

  HDAC 1 antibodyHDAC1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 antibodyRPD3L1 antibody

  see all

Anti-HDAC1 antibody [EPR460(2)] images

• 

  Western blot - HDAC1 antibody [EPR460(2)] (ab109411)
  All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : HeLa cell lysate
  Lane 3 : K562 cell lysate
  Lane 4 : MCF7 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 55 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)
  Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.
• 

  Immunocytochemistry/ Immunofluorescence - Anti-HDAC1 antibody [EPR460(2)] (ab109411)Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
  ab109411 (1/400) staining HDAC1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.

  See Abreview

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody [EPR460(2)] (ab109411)
  Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.