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Overview

  • Product nameAnti-HDAC1 antibody [HDAC1-21]See all HDAC1 primary antibodies ...
  • Description
    Mouse monoclonal [HDAC1-21] to HDAC1
  • Tested applicationsELISA, IP, WB more details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat
  • Immunogen

    Synthetic peptide: KEEKPEAKGVKEEVKLA, corresponding to amino acids 466-482 of Human HDAC1.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive controlHeLa cell whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab12168 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
IP IP: Use at an assay dependent dilution.
WB WB: Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 65 kDa.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q13547 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody
      HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody
    see all

References for Anti-HDAC1 antibody [HDAC1-21] (ab12168)

This product has been referenced in:
  • Wang AG  et al. Hepatic steatosis in transgenic mice overexpressing human histone deacetylase 1. Biochem Biophys Res Commun 330:461-6 (2005). WB ; Mouse . Read more (PubMed: 15796905) »

See 1 Publication for this product

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A customer has asked for confirmation as to whether ab12168, HDAC1 antibody [HDAC1-21] and ab9326, c-Myc antibody [3C7 ] will detect both reduced and denatured protein in western blot. I've looked at the data sheet but I was hoping you could provide ...

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Thank you for your enquiry. Please aaccept my apologies for the delay to your enquiry. I have found it difficult to find much information concerning the conditions employed using ab9326 in western blotting. However, in the following reference they refe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"