Anti-HDAC1 antibody (ab19845)

Overview

• Product nameAnti-HDAC1 antibodySee all HDAC1 primary antibodies ...
• Description
  Rabbit polyclonal to HDAC1
• Tested applicationsICC/IF, WB, IP, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  Predicted to work with: Cow
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab20434.)

• Positive controlThis antibody gave a positive signal in Hela whole cell lysate and Human Breast Carcinoma.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
• Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
• Post-translational
  modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
  Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
  Ubiquitinated by CHFR, leading to its degradation by the proteasome.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 404126 Cow
  • Entrez Gene: 3065 Human
  • Entrez Gene: 433759 Mouse
  • Entrez Gene: 297893 Rat
  • Omim: 601241 Human
  • SwissProt: Q32PJ8 Cow
  • SwissProt: Q13547 Human
  • SwissProt: O09106 Mouse

  • SwissProt: Q4QQW4 Rat
  • Unigene: 88556 Human
  • Unigene: 202504 Mouse
  • Unigene: 391033 Mouse
  • Unigene: 1863 Rat

  see all

• Alternative names
  DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody

  HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody

  see all

Anti-HDAC1 antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC1 antibody (ab19845)
  The image shows staining of human tonsil tissue using ab19845. Staining was nuclear and was equally successful using Tris EDTA pH9 or Citrate pH6 for antigen retrieval. Staining was prevalent in almost all cellular compartments of the tonsil.
• 

  Western blot - HDAC1 antibody (ab19845)
  All lanes : Anti-HDAC1 antibody (ab19845) at 1 µg/ml
  
  Lane 1 : HeLa whole cell lysate
  Lane 2 : HeLa whole cell lysate with HDAC1 peptide (ab20434) at 1 µg/ml
  
  Lysates/proteins at 20 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab7090) at 1/5000 dilution
  
  Predicted band size : 55 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - HDAC1 antibody (ab19845)

  ICC/IF image of ab19845 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab19845, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iT^TM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  Panel A shows localisation of ab19845 to the nuclei, Panel B has the Alexa Fluor® 488 channel removed for comparison.

• 

  Immunocytochemistry/ Immunofluorescence - HDAC1 antibody (ab19845)

  ab19845 at a 1/3000 dilution staining asynchronous HeLa cells by ICC/IF. The cells were paraformaldehyde fixed and immunofluorescently labelled with ab19845 for 30 minutes at room temperature. Bound antibody was detected using a Cy3 conjugated goat anti-rabbit antibody. Nuclei were visuallised using DAPI staining. The antibody was found to be highly enriched in the nucleus.

  This image is courtesy of an Abreview submitted by Kirk McManus.

  See Abreview

• 

  Immunodiffusion - HDAC1 antibody (ab19845)
  ICC/IF image of ab19845 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab19845, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2 cells at 1µg/ml, and in 100% methanol fixed (5 min) MCF7 and HepG2 cells at 1µg/ml
• 

  Immunocytochemistry/ Immunofluorescence - HDAC1 antibody (ab19845)
  IHC image of HDAC1 staining in human breast carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19845, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX
• 

  Immunoprecipitation - Anti-HDAC1 antibody (ab19845)
  HDAC1 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to HDAC1 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
  The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
  Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70^oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab19845.
  Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
  Band: 60ka: HDAC1.