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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Zebrafish HDAC1.
(Peptide available as ab41405.)
Our Abpromise guarantee covers the use of ab33278 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.06 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Zebrafish HDAC1 peptide (ab41405) or Zebrafish HDAC1 peptide (ab41406).|
|ICC/IF||Use a concentration of 1 µg/ml.|
ICC/IF image of ab33278 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33278, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.
Immunohistochemistical detection of HDAC1 using antibody ab33278 on PFA perfusion fixed frozen rat heart sections. Primary antibody Dilution 1/300, Incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor 488 (1/1000). The antibody produced some staining in the cytoplasm and what seems to be the nucleus of muscle cells in rat heart tissue. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.
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