Overview

  • Product nameAnti-HDAC1 antibodySee all HDAC1 primary antibodies ...
  • Description
    Rabbit polyclonal to HDAC1
  • Tested applicationsIHC-FoFr, WB, ICC/IF more details
  • Species reactivity
    Reacts with: Rat, Human, Xenopus laevis, Zebrafish
    Predicted to work with: Mouse, Chicken, Cow
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Zebrafish HDAC1.

    (Peptide available as ab41405.)

  • Positive control
    • This antibody gave a positive signal in the HeLa, HEK293 and MCF7 whole cell lysates as well as Zebrafish Embryo Extract.

Properties

Applications

Our Abpromise guarantee covers the use of ab33278 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-FoFr 1/300.
WB Use a concentration of 0.06 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 56 kDa).Can be blocked with Zebrafish HDAC1 peptide (ab41405) or Zebrafish HDAC1 peptide (ab41406).
ICC/IF Use a concentration of 1 µg/ml.

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications
    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.
  • Target information above from: UniProt accession Q13547 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • DKFZp686H12203 antibody
    • GON 10 antibody
    • HD 1 antibody
    • HD1 antibody
    • HDAC 1 antibody
    • HDAC1 antibody
    • hdac1: histone deacetylase 1 antibody
    • HDAC1_HUMAN antibody
    • Histone deacetylase 1 (HD1) antibody
    • Histone deacetylase 1 antibody
    • Reduced potassium dependency yeast homolog like 1 antibody
    • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
    • RPD3 (reduced potassium dependency) antibody
    • RPD3 antibody
    • RPD3L1 antibody
    see all

Anti-HDAC1 antibody images

  • All lanes : Anti-HDAC1 antibody (ab33278) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 3 : Zebrafish Embryo Extract

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 56 kDa


    Exposure time : 1 minute
  • Anti-HDAC1 antibody (ab33278) at 0.06 µg/ml + whole embryo zebrafish tissue at 45 µg

    Secondary
    whole embryo zebrafish tissue lysate at 1/5000 dilution

    Predicted band size : 56 kDa
    Observed band size : 56 kDa
    Additional bands at : 32 kDa (possible cross reactivity),37 kDa (possible cross reactivity).

    See Abreview

  • ICC/IF image of ab33278 stained human MCF7 cells. The cells were methanol fixed (5 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab33278, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HeLa, HEK 293 and HepG2 cells.

  • Immunohistochemistical detection of HDAC1 using antibody ab33278 on PFA perfusion fixed frozen rat heart sections. Primary antibody Dilution 1/300, Incubated for 18 hours @ 20°C in PBS + 0.3 % Triton X100. Secondary antibody: Goat anti-rabbit Alexa Fluor 488 (1/1000). The antibody produced some staining in the cytoplasm and what seems to be the nucleus of muscle cells in rat heart tissue. The tissues were fixed (animals perfused fixed) with 4% PFA and later postfixed overnight in the same fixative. They were cryoprotected in 30% sucrose and cut using a cryostat.

    See Abreview

References for Anti-HDAC1 antibody (ab33278)

This product has been referenced in:
  • Ignatius MS  et al. Distinct functional and temporal requirements for zebrafish Hdac1 during neural crest-derived craniofacial and peripheral neuron development. PLoS One 8:e63218 (2013). WB ; Zebrafish . Read more (PubMed: 23667588) »

See 1 Publication for this product

Product Wall

We do not have IF data for these two species, just the western blot data we received from customers. Here are the two links to the blot data.

Zebrafish
http://www.abcam.com/HDAC1-antibody-ab33278-reviews.html?intabreviewid=9620
Read More

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Rat Tissue sections (Heart)
Specification Heart
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Username

Dr. Sophie Pezet

Verified customer

Submitted Mar 22 2010

Application Western blot
Sample Xenopus laevis Cell lysate - whole cell (Egg extract)
Loading amount 75 µg
Specification Egg extract
Gel Running Conditions Reduced Denaturing (8%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Jul 10 2009

Application Western blot
Sample Zebrafish Tissue lysate - whole (whole embryo)
Loading amount 45 µg
Specification whole embryo
Gel Running Conditions Reduced Denaturing
Blocking step Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
Username

Abcam user community

Verified customer

Submitted Jan 18 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"