Anti-HDAC1 antibody (ab53091)

Overview

• Product nameAnti-HDAC1 antibodySee all HDAC1 primary antibodies ...
• Description
  Rabbit polyclonal to HDAC1
• Specificityab53091 detects endogenous levels of total HDAC1 protein.
• Tested applicationsFlow Cyt, ICC, WB, IHC-P, ELISA, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide derived from human HDAC1

• Positive controlWB: NIH/3T3 cell extracts IHC-P: Human lung carcinoma tissue

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab53091 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
• Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
• Post-translational
  modificationsSumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
  Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
  Ubiquitinated by CHFR, leading to its degradation by the proteasome.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3065 Human
  • Entrez Gene: 433759 Mouse
  • Entrez Gene: 297893 Rat
  • Omim: 601241 Human
  • SwissProt: Q13547 Human
  • SwissProt: O09106 Mouse
  • SwissProt: Q4QQW4 Rat
  • Unigene: 88556 Human

  • Unigene: 202504 Mouse
  • Unigene: 391033 Mouse
  • Unigene: 1863 Rat

  see all

• Alternative names
  DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody

  HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody

  see all

Anti-HDAC1 antibody images

• 

  Western blot - HDAC1 antibody (ab53091)
  All lanes : Anti-HDAC1 antibody (ab53091) at 1/300 dilution
  
  Lane 1 : NIH/3T3 cell extracts
  Lane 2 : NIH/3T3 cell extracts with blocking peptide
  
  
  Predicted band size : 55 kDa
  Observed band size : 60 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Paraffin-embedded sections) - HDAC1 antibody (ab53091)
  Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using ab53091 at 1/50 dilution, in the presence (right) or absence (left) of blocking peptide.
• 

  Immunocytochemistry/ Immunofluorescence-HDAC1 antibody(ab53091)
  ICC/IF image of ab53091 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53091, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.