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Related products
- Secondary antibodies
- Lysates
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Product name
Anti-HDAC1 antibody
See all HDAC1 products (22) ...
Description
Rabbit polyclonal to HDAC1
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the N-terminus of human HDAC1
Positive control
Whole cell lysate from HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7 to 8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70494 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP
Application notes
IP: Use at 2 - 5 µg/mg of lysate.
Is unsuitable for WB.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
Tissue specificity
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ13547
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686H12203 antibody
- GON 10 antibody
- HD 1 antibody
see all
Database links
- Entrez Gene: 3065 Human
- Omim: 601241 Human
- SwissProt: Q13547 Human
- Unigene: 88556 Human
References for Anti-HDAC1 antibody (ab70494)
ab70494 has not yet been referenced specifically in any publications.
Publishing research using ab70494? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"