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Anti-HDAC1 antibody [mAbcam 68436] (ab68436)

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Overview

  • Product nameAnti-HDAC1 antibody [mAbcam 68436]See all HDAC1 primary antibodies ...
  • Description
    Mouse monoclonal [mAbcam 68436] to HDAC1
  • Tested applicationsWB more details
  • Species reactivity
    Reacts with: Mouse
    Predicted to work with: Rat, Cow, Human, Orangutan
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC1.

    Read Abcam's proprietary immunogen policy

    (Peptide available as ab20434.)

  • Positive controlThis antibody gave a positive signal in IOUD2 Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab68436 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: Use a concentration of 5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).Can be blocked with HDAC1 peptide (ab20434).

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
  • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
  • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
  • Post-translational
    modifications    Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
    Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
    Ubiquitinated by CHFR, leading to its degradation by the proteasome.
  • Cellular localizationNucleus.

    • Target information above from: UniProt accession Q13547 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody
      HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody
    see all

Anti-HDAC1 antibody [mAbcam 68436] images

  • Western blot - HDAC1 antibody (ab68436)
    Western blot - HDAC1 antibody (ab68436)
    Anti-HDAC1 antibody [mAbcam 68436] (ab68436) at 5 µg/ml + IOUD2 Whole Cell Lysate at 20 µg

    Secondary
    Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 55 kDa
    Observed band size : 60 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute

References for Anti-HDAC1 antibody [mAbcam 68436] (ab68436)

ab68436 has not yet been referenced specifically in any publications.

Publishing research using ab68436 ? Please let us know so that we can cite the reference in this datasheet

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Immunohistochemistry (Frozen sections) abreview for Anti-HDAC1 antibody [mAbcam 68436]

 Inconclusive
Abreviews
Application Immunohistochemistry (Frozen sections)
Sample Rat Tissue sections (Spinal cord)
Specification Spinal cord
Fixative Paraformaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
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Submitted Aug 24 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"