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Overview

  • Product nameAnti-HDAC1 antibody, predilutedSee all HDAC1 primary antibodies ...
  • Description
    Rabbit polyclonal to HDAC1, prediluted
  • Tested applicationsIHC-P more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

  • Positive controlNIH/3T3 cells or Tonsil

Properties

Applications

Our Abpromise guarantee covers the use of ab15316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P
  • Application notesIHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
    • Tissue specificityUbiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
    • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications      Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
      Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
      Ubiquitinated by CHFR, leading to its degradation by the proteasome.
    • Cellular localizationNucleus.

      • Target information above from: UniProt accession Q13547 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
      see all
    • Alternative names
        DKFZp686H12203 antibodyGON 10 antibodyHD 1 antibody
        HD1 antibodyHDAC 1 antibodyHDAC1 antibodyhdac1: histone deacetylase 1 antibodyHDAC1_HUMAN antibodyHistone deacetylase 1 (HD1) antibodyHistone deacetylase 1 antibodyReduced potassium dependency yeast homolog like 1 antibodyRPD3 (reduced potassium dependency yeast homolog) like 1 antibodyRPD3 (reduced potassium dependency) antibodyRPD3 antibodyRPD3L1 antibody
      see all

    Anti-HDAC1 antibody, prediluted images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)
      ab15316 staining HDAC1 in breast carcinoma by Immunohistochemistry (FFPE-sections).
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)Image from Samartzis N et al., BMC Cancer. 2011 Oct 26;11:463. Fig 1.; doi:10.1186/1471-2407-11-463; 26 October 2011, BMC Cancer 2011, 11:463
      Immunohistochemical analysis of Human vulvar squamous cell cancer, staining HDAC1 with ab15316 at 1/2 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)Image from Samartzis N et al., BMC Cancer. 2011 Oct 26;11:463. Fig 1.; doi:10.1186/1471-2407-11-463; 26 October 2011, BMC Cancer 2011, 11:463
      Immunohistochemical analysis of Human vulvar intraepithelial neoplasia, staining HDAC1 with ab15316 at 1/2 dilution.

    References for Anti-HDAC1 antibody, prediluted (ab15316)

    This product has been referenced in:
    • Quint K  et al. Clinical significance of histone deacetylases 1, 2, 3, and 7: HDAC2 is an independent predictor of survival in HCC. Virchows Arch 459:129-39 (2011). IHC-P ; Human . Read more (PubMed: 21713366) »
    • Samartzis N  et al. Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study. BMC Cancer 11:463 (2011). IHC-P . Read more (PubMed: 22029821) »

    See all 5 Publications for this product

    Publishing research using ab15316 ? Please let us know so that we can cite the reference in this datasheet

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    10
    Votes: Highest First

    I recently order one of your product, the rabbit polyclonal antibody anti-HDAC1 (ab15316) and I have a question regarding the immunogen. I know the exact immunogen sequence is commercially sensitive, but I would like to know if you could tell me wha...

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    Thank you for your message. I can confirm that the alignment of the immunogen for ab15316 HDAC1 antibody with HDAC 2,3,6,and 10 is as follows: HDAC2 54.50% HDAC3 35.70% HDAC6 0% HDAC10 0% These alignments are very low and so I would sugg...

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