You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-HDAC1 antibody, prediluted (ab15316)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-HDAC1 antibody, prediluted
See all HDAC1 products (22) ...

Description

Rabbit polyclonal to HDAC1, prediluted

Tested applications

IHC-Pmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide (unfortunately, the amino acid sequence is considered to be commercially sensitive) (Human) (C terminal).

Positive control

NIH/3T3 cells or Tonsil

Properties

Form

Prediluted

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 50mM Tris, pH 7.6

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab15316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

IHC-P: Use at an assay dependent dilution. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10 min followed by cooling at RT for 20 min.

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.

Tissue specificity

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DKFZp686H12203 antibody
  • GON 10 antibody
  • HD 1 antibody
  • HD1 antibody
  • HDAC 1 antibody
  • HDAC1 antibody
  • hdac1: histone deacetylase 1 antibody
  • HDAC1_HUMAN antibody
  • Histone deacetylase 1 (HD1) antibody
  • Histone deacetylase 1 antibody
  • Reduced potassium dependency yeast homolog like 1 antibody
  • RPD3 (reduced potassium dependency yeast homolog) like 1 antibody
  • RPD3 (reduced potassium dependency) antibody
  • RPD3 antibody
  • RPD3L1 antibody
see all

Database links

see all

Anti-HDAC1 antibody, prediluted images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody, prediluted (ab15316)

ab15316 staining HDAC1 in breast carcinoma by Immunohistochemistry (FFPE-sections).

References for Anti-HDAC1 antibody, prediluted (ab15316)

This product has been referenced in:

  • Quint K  et al. Clinical significance of histone deacetylases 1, 2, 3, and 7: HDAC2 is an independent predictor of survival in HCC. Virchows Arch 459:129-39 (2011). IHC-P; Human.Read more (PubMed: 21713366) »
  • Samartzis N  et al. Expression pattern of class I histone deacetylases in vulvar intraepithelial neoplasia and vulvar cancer: a tissue microarray study. BMC Cancer 11:463 (2011). IHC-P.Read more (PubMed: 22029821) »

See all 5 publications for this product

Publishing research using ab15316? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"