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Product name
Anti-HDAC10 antibody - Aminoterminal end
See all HDAC10 products (7) ...
Description
Rabbit polyclonal to HDAC10 - Aminoterminal end
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Immunogen
A KLH conjugated synthetic peptide selected from a region of human HDAC10 within amino acids 1-100.
Positive control
A375 cell line lysate and 293 cell lysate transiently transfected with the HDAC10 gene.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab71215 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/50 - 1/100. Detects a band of approximately 71 kDa (predicted molecular weight: 71 kDa).
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Tissue specificity
Ubiquitous. High expression in liver, spleen, pancreas and kidney.
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily.
Cellular localization
Cytoplasm. Nucleus. Excluded from the nucleoli.
Target information above from: UniProt accessionQ969S8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZP761B039 antibody
- HD 10 antibody
- HD10 antibody
see all
Database links
- Entrez Gene: 83933 Human
- Omim: 608544 Human
- SwissProt: Q969S8 Human
- Unigene: 26593 Human
Anti-HDAC10 antibody - Aminoterminal end images:
Western blot - HDAC10 antibody - Aminoterminal end (ab71215)
Anti-HDAC10 antibody - Aminoterminal end (ab71215) at 1/100 dilution + A375 cell line lysates at 12.5 µg
Predicted band size : 71 kDa
Observed band size : 71 kDa
Additional bands at : 150 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Western blot - HDAC10 antibody - Aminoterminal end (ab71215)
All lanes : Anti-HDAC10 antibody - Aminoterminal end (ab71215) at 1/100 dilution
Lane 1 : 293 cell lysates non transfected
Lane 2 : 293 cell lysates transiently transfected with the HDAC10 gene
Lysates/proteins at 2 µg per lane.
Predicted band size : 71 kDa
Observed band size : 71 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
References for Anti-HDAC10 antibody - Aminoterminal end (ab71215)
ab71215 has not yet been referenced specifically in any publications.
Publishing research using ab71215? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"