Overview
- Product nameAnti-HDAC11 antibodySee all HDAC11 primary antibodies ...
- DescriptionRabbit polyclonal to HDAC11
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow, Rhesus monkey - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HDAC11.
(Peptide available as ab98895.)
- Positive controlThis antibody gave a positive signal in Human Skeletal Muscle Tissue Lysate as well as HeLa and NIH3T3 Whole Cell Lysates.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab87765 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with HDAC11 peptide (ab98895). |
Target
- FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
- Tissue specificityWeakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
- Sequence similaritiesBelongs to the histone deacetylase family.
- Cellular localizationNucleus. Predominantly nuclear.
-
Database links
- Entrez Gene: 79885 Human
- Entrez Gene: 232232 Mouse
- Entrez Gene: 297453 Rat
- Omim: 607226 Human
- SwissProt: Q96DB2 Human
- SwissProt: Q91WA3 Mouse
- Unigene: 728246 Human
- Unigene: 744132 Human
- Unigene: 206218 Mouse
- Unigene: 28065 Rat
see all
Target information above from: UniProt accession
Q96DB2
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- FLJ22237 antibodyHD 11 antibodyHD11 antibody
- HDA11_HUMAN antibodyHDAC 11 antibodyHDAC11 antibodyHistone deacetylase 11 antibody
see all
Anti-HDAC11 antibody images
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Western blot - Anti-HDAC11 antibody (ab87765)All lanes : Anti-HDAC11 antibody (ab87765) at 1 µg/ml
Lane 1 :Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 37 kDa
Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 20 minutes -
Western blot - Anti-HDAC11 antibody (ab87765)Anti-HDAC11 antibody (ab87765) at 1 µg/ml +HDAC11 protein (ab101113) at 0.1 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Exposure time : 1 minute -
Western blot - Anti-HDAC11 antibody (ab87765)Anti-HDAC11 antibody (ab87765) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 37 kDa
Observed band size : 37 kDa
Exposure time : 20 minutes
References for Anti-HDAC11 antibody (ab87765)
ab87765 has not yet been referenced specifically in any publications.
