Overview

  • Product nameAnti-HDAC11 antibody
    See all HDAC11 primary antibodies
  • Description
    Rabbit polyclonal to HDAC11
  • Tested applicationsWBmore details
  • Species reactivity
    Reacts with: Mouse, Human
    Predicted to work with: Rat, Cow, Rhesus monkey
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HDAC11.

    (Peptide available as ab98895.)

  • Positive control
    • This antibody gave a positive signal in Human Skeletal Muscle Tissue Lysate as well as HeLa and NIH3T3 Whole Cell Lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab87765 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 37 kDa).Can be blocked with Human HDAC11 peptide (ab98895).

Target

  • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
  • Tissue specificityWeakly expressed in most tissues. Strongly expressed in brain, heart, skeletal muscle, kidney and testis.
  • Sequence similaritiesBelongs to the histone deacetylase family.
  • Cellular localizationNucleus. Predominantly nuclear.
  • Information by UniProt
  • Database links
  • Alternative names
    • FLJ22237 antibody
    • HD 11 antibody
    • HD11 antibody
    • HDA11_HUMAN antibody
    • HDAC 11 antibody
    • HDAC11 antibody
    • Histone deacetylase 11 antibody
    see all

Anti-HDAC11 antibody images

  • All lanes : Anti-HDAC11 antibody (ab87765) at 1 µg/ml

    Lane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)
    Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa
    Additional bands at : 52 kDa,60 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes
  • Anti-HDAC11 antibody (ab87765) at 1 µg/ml + Human HDAC11 full length protein (ab101113) at 0.1 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 1 minute
  • Anti-HDAC11 antibody (ab87765) at 1 µg/ml + NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 37 kDa
    Observed band size : 37 kDa


    Exposure time : 20 minutes

References for Anti-HDAC11 antibody (ab87765)

ab87765 has not yet been referenced specifically in any publications.

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