Anti-HDAC2 antibody [EPR5001] (ab124974)

Overview

• Product nameAnti-HDAC2 antibody [EPR5001]See all HDAC2 primary antibodies ...
• Description
  Rabbit monoclonal [EPR5001] to HDAC2
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues on the N-terminus in Human HDAC2 protein (Q92769).

• Positive controlHeLa, SH-SY5Y, Jurkat, and A431 cell lysates.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferpH: 7.40
  Preservative: 0.01% Sodium azide
  Constituents: 50% Glycerol, 0.05% BSA
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR5001
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
  Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
• Tissue specificityWidely expressed; lower levels in brain and lung.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
• Post-translational
  modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q92769 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 3066 Human
  • Entrez Gene: 15182 Mouse
  • Entrez Gene: 84577 Rat
  • Omim: 605164 Human
  • SwissProt: Q92769 Human
  • SwissProt: P70288 Mouse
  • Unigene: 3352 Human
  • Unigene: 19806 Mouse

  • Unigene: 1797 Rat

  see all

• Alternative names
  D10Wsu179e antibodyHD 2 antibodyHD2 antibody

  HDAC 2 antibodyHdac2 antibodyHDAC2_HUMAN antibodyHistone deacetylase 2 (HD2) antibodyHistone deacetylase 2 antibodyOTTHUMP00000017046 antibodyOTTHUMP00000227077 antibodyOTTHUMP00000227078 antibodyRPD3 antibodytranscriptional regulator homolog RPD3 antibodyYAF1 antibodyYY1 associated factor 1 antibodyYY1 transcription factor binding protein antibodyYy1bp antibody

  see all

Anti-HDAC2 antibody [EPR5001] images

• 

  Western blot - Anti-HDAC2 antibody [EPR5001] (ab124974)
  All lanes : Anti-HDAC2 antibody [EPR5001] (ab124974) at 1/10000 dilution
  
  Lane 1 : HeLa cell lysate
  Lane 2 : SH-SY5Y cell lysate
  Lane 3 : Jurkat cell lysate
  Lane 4 : A431 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Predicted band size : 55 kDa