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Anti-HDAC2 antibody [Y461] (ab32117)

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Overview

Product name

Anti-HDAC2 antibody [Y461]
See all HDAC2 products (12) ...

Description

Rabbit monoclonal [Y461] to HDAC2

Specificity

ab32117 recognises HDAC2.

Tested applications

WB, IHC-P, ICC/IF, Flow Cyt, IP, IHC-Frmore details

Cross reactivity

Reacts with

Mouse, Rat, Human

Immunogen

Synthetic peptide corresponding to residues in the C terminal of human HDAC2.

Positive control

Human breast carcinoma, K562 cells, HeLa cells.

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Concentration

Concentration information loading...

Clonality

Monoclonal

Clone number

Y461

Isotype

IgG

Research areas

Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Flow Cytometry-HDAC2 antibody [Y461](ab32117)Flow Cytometry-HDAC2 antibody [Y461](ab32117) image (enlarge)

  • Western blot - HDAC2 antibody [Y461] (ab32117)Western blot - HDAC2 antibody [Y461] (ab32117) image (enlarge)

  • Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461] (ab32117)Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461] (ab32117) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab32117 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.

Tissue specificity

Widely expressed; lower levels in brain and lung.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.

Cellular localization

Nucleus.

Target information above from: UniProt accessionQ92769 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • D10Wsu179e antibody
  • HD 2 antibody
  • HD2 antibody
  • HD2 antibody
  • HDAC 2 antibody
  • Hdac2 antibody
  • HDAC2_HUMAN antibody
  • Histone deacetylase 2 (HD2) antibody
  • Histone deacetylase 2 (HD2) antibody
  • histone deacetylase 2 antibody
  • histone deacetylase 2 antibody
  • OTTHUMP00000017046 antibody
  • OTTHUMP00000227077 antibody
  • OTTHUMP00000227078 antibody
  • RPD3 antibody
  • RPD3 antibody
  • transcriptional regulator homolog RPD3 antibody
  • transcriptional regulator homolog RPD3 antibody
  • YAF1 antibody
  • YY1 associated factor 1 antibody
  • YY1 transcription factor binding protein antibody
  • Yy1bp antibody
see all

Database links

Anti-HDAC2 antibody [Y461] images:

  Flow Cytometry-HDAC2 antibody [Y461](ab32117)

Flow Cytometry-HDAC2 antibody [Y461](ab32117)

Overlay histogram showing HeLa cells stained with ab32117 (red line). The cells were fixed with methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab32117, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a decreased signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.

  Western blot - HDAC2 antibody [Y461] (ab32117)

Western blot - HDAC2 antibody [Y461] (ab32117)

Anti-HDAC2 antibody [Y461] (ab32117) at 1/2000 dilution + K562 cell lysate

Predicted band size : 55 kDa
Observed band size : 70 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461] (ab32117)

Immunohistochemistry (Paraffin-embedded sections) - HDAC2 antibody [Y461] (ab32117)

Immunohistochemical analysis of HDAC2 expression in paraffin embedded human breast carcinoma tissue section, using 1/250 ab32117.

  Immunocytochemistry/ Immunofluorescence - HDAC2 antibody [Y461] (ab32117)

Immunocytochemistry/ Immunofluorescence - HDAC2 antibody [Y461] (ab32117)

Immunofluorescent analysis of HDAC expression in HeLa cells, using 1/250 ab32117.

  Immunohistochemistry (Frozen sections) - HDAC2 antibody [Y461] (ab32117)

Immunohistochemistry (Frozen sections) - HDAC2 antibody [Y461] (ab32117)

ab32117 staining HDAC2 in Rat spinal cord tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 1% BSA for 30 minutes at 25°C. Samples were incubated with primary antibody (1/500 in PBS + 0.2% TritonX + 1% BSA) for 16 hours at 4°C. An Alexa Fluor®488-conjugated Donkey anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Antigen unmasking with sodium citrate buffer (10mM sodium citrate, 0.05% Tween 20, ph6) was necessary to obtain a good signal. The sections were counterstained with DAPI.

This image is courtesy of an anonymous Abreview

See Abreview

References for Anti-HDAC2 antibody [Y461] (ab32117)

This product has been referenced in:

  • Martinez FP & Tang Q The leucine zipper domain is required for the Kaposi Sarcoma-associated herpesvirus (KSHV) K-bZIP protein to interact with histone deacetylase and is important for KSHV replication. J Biol Chem : (2012).Read more (PubMed: 22416134) »
  • Hnilicová J  et al. Histone deacetylase activity modulates alternative splicing. PLoS One 6:e16727 (2011). WB; Human.Read more (PubMed: 21311748) »

See all 8 publications for this product

Publishing research using ab32117? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"