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Product name
Anti-HDAC2 antibody
See all HDAC2 products (12) ...
Description
Rabbit polyclonal to HDAC2
Tested applications
IHC-Fr, IHC-P, ICC/IF, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Does not react with
Chicken
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human HDAC2.
(Peptide available as ab162 00.)
Positive control
This antibody gave a positive signal in the following whole cell lysates: Hela; A431; Jurkat; HEK293 and Hela Nuclear lysate.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Heart >> Hypertrophy >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16032 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-Fr: 1/500.
IHC-P: 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF: Use a concentration of 1 µg/ml.
WB: Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 55.3 kDa).
IP: Use at an assay dependent concentration.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity.
Tissue specificity
Widely expressed; lower levels in brain and lung.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
S-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching.
Cellular localization
Nucleus.
Target information above from: UniProt accessionQ92769
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
see all
Database links
- Entrez Gene: 3066 Human
- Entrez Gene: 15182 Mouse
- Entrez Gene: 84577 Rat
- Omim: 605164 Human
- SwissProt: Q92769 Human
- SwissProt: P70288 Mouse
- Unigene: 3352 Human
- Unigene: 19806 Mouse
Anti-HDAC2 antibody images:
Western blot - HDAC2 antibody (ab16032)
All lanes : Anti-HDAC2 antibody (ab16032) at 0.4 µg/ml
Lane 1 : HeLa nuclear lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HeLa nuclear lysate with
Lane 7 : HeLa whole cell lysate with
Lane 8 : A431 cell lysate with
Lane 9 : Jurkat cell lysate with
Lane 10 : HEK293 cell lysate with
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated Goat anti-Rabbit IgG at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55.3 kDa
Exposure time : 30 seconds
ab16032 detects a band at approximately 50kDa and a weaker band at approximately 60kDa. We believe the ~ 50kDa band represents HDAC2.
Immunoprecipitation - Anti-HDAC2 antibody (ab16032)
HDAC2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to HDAC2 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16032. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 60ka: HDAC2.
Immunocytochemistry/ Immunofluorescence - HDAC2 antibody (ab16032)
ICC/IF image of ab16032 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab16032, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 phalloidin was used to label F-actin (red).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC2 antibody (ab16032)
ab16032 (1/250) staining HDAC2 in paraffin-embedded Human tonsil tissue. Tissue underwent fixation in formaldehyde, peroxidase blocking, protein blocking and heat mediated antigen retrieval. The secondary antibody was goat anti rabbit/mouse conjugated to HRP. For further experimental details please refer to abreview.
This image is courtesy of an abreview submitted by Antibody Solutions Ltd.
Immunohistochemistry (Frozen sections) - HDAC2 antibody (ab16032)
IHC-Fr image of HDAC2 (ab16032) staining on Rat spinal cord. The sections required antigen retrieval with sodium citrate buffer was necessary to obtain full signal strength (sodium citrate 10mM, pH6)
Image courtsey Anonymus Abreview
Western blot - HDAC2 antibody (ab16032)
All lanes : Anti-HDAC2 antibody (ab16032) at 0.4 µg/ml
Lane 1 : Mouse 3T3 lysate
Lane 2 : Rat liver lysate
Lane 3 : Chicken liver lysate
Lysates/proteins at 20 µg/ml per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 55.3 kDa
Observed band size : 50,60 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
ab16032 cross-reacts with Mouse 3T3 cells but shows no cross-reactivity against either Rat liver or Chicken cell lysates.
References for Anti-HDAC2 antibody (ab16032)
This product has been referenced in:
- Lee TK et al. An N-terminal truncated carboxypeptidase E splice isoform induces tumor growth and is a biomarker for predicting future metastasis in human cancers. J Clin Invest : (2011). WB; Human.Read more (PubMed: 21285511) »
- Smith JA et al. A role for the histone deacetylase HDAC4 in the life-cycle of HIV-1-based vectors. Virol J 7:237 (2010). ChIP; Human.Read more (PubMed: 20846395) »
See all 6 publications for this product
Publishing research using ab16032? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"