Anti-HDAC2 (phospho S394) antibody (ab75602)
Key features and details
- Rabbit polyclonal to HDAC2 (phospho S394)
- Suitable for: ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HDAC2 (phospho S394) antibody
See all HDAC2 primary antibodies -
Description
Rabbit polyclonal to HDAC2 (phospho S394) -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human HDAC2 (phospho S394).
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Positive control
- Extracts from HT-29 cells treated with UV (20min). Human breast carcinoma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab75602 was affinity-purified from rabbit antiserum by affinity chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab75602 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/100 - 1/200.
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WB | (1) |
1/500 - 1/1000. Predicted molecular weight: 55 kDa.
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IHC-P | (1) |
1/50 - 1/100.
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Notes |
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ICC/IF
1/100 - 1/200. |
WB
1/500 - 1/1000. Predicted molecular weight: 55 kDa. |
IHC-P
1/50 - 1/100. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes.
Forms transcriptional repressor complexes by associating with MAD, SIN3, YY1 and N-COR. Interacts in the late S-phase of DNA-replication with DNMT1 in the other transcriptional repressor complex composed of DNMT1, DMAP1, PCNA, CAF1. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. -
Tissue specificity
Widely expressed; lower levels in brain and lung. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsS-nitrosylated by GAPDH. In neurons, S-Nitrosylation at Cys-262 and Cys-274 does not affect the enzyme activity but abolishes chromatin-binding, leading to increases acetylation of histones and activate genes that are associated with neuronal development. In embryonic cortical neurons, S-Nitrosylation regulates dendritic growth and branching. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3066 Human
- Omim: 605164 Human
- SwissProt: Q92769 Human
- Unigene: 3352 Human
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Alternative names
- D10Wsu179e antibody
- HD 2 antibody
- HD2 antibody
see all
Images
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All lanes : Anti-HDAC2 (phospho S394) antibody (ab75602) at 1/500 dilution
Lane 1 : Extracts from HT-29 cells (untreated)
Lane 2 : Extracts from HT-29 cells treated with UV (20min)
Predicted band size: 55 kDa
Observed band size: 55 kDa -
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab75602, at 1/50 dilution, in the absence (left panel) and presence (right panel) of the immunising phosphopeptide.
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ab75602 at 1/100 dilution, staining HDAC2 (phospho S394) in human Hela cells by ICC/IF.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab75602 has been referenced in 7 publications.
- Huegel J et al. CUDC907, a dual phosphoinositide-3 kinase/histone deacetylase inhibitor, promotes apoptosis of NF2 Schwannoma cells. Oncotarget 13:890-904 (2022). PubMed: 35875610
- Yu Z et al. Enriched environment improves sevoflurane-induced cognitive impairment during late-pregnancy via hippocampal histone acetylation. Braz J Med Biol Res 53:e9861 (2020). PubMed: 32813852
- Shang L et al. Plantamajoside attenuates isoproterenol-induced cardiac hypertrophy associated with the HDAC2 and AKT/ GSK-3ß signaling pathway. Chem Biol Interact 307:21-28 (2019). PubMed: 31009642
- Mattioli E et al. Altered modulation of lamin A/C-HDAC2 interaction and p21 expression during oxidative stress response in HGPS. Aging Cell 17:e12824 (2018). PubMed: 30109767
- Yoon S et al. PP2A negatively regulates the hypertrophic response by dephosphorylating HDAC2 S394 in the heart. Exp Mol Med 50:83 (2018). PubMed: 30050113
- Kobayashi Y et al. Passive smoking impairs histone deacetylase-2 in children with severe asthma. Chest 145:305-12 (2014). Human . PubMed: 24030221
- Khan DH et al. RNA-dependent dynamic histone acetylation regulates MCL1 alternative splicing. Nucleic Acids Res N/A:N/A (2013). PubMed: 24234443