Anti-HDAC3 antibody [3G6] - C-terminal (ab170199)
Key features and details
- Mouse monoclonal [3G6] to HDAC3 - C-terminal
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG2a
Overview
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Product name
Anti-HDAC3 antibody [3G6] - C-terminal
See all HDAC3 primary antibodies -
Description
Mouse monoclonal [3G6] to HDAC3 - C-terminal -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human HDAC3 aa 411-428 (C terminal).
Sequence:NEFYDGDHDNDKESDVEI
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Positive control
- mouse NIH 3T3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.1% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
3G6 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab170199 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/100 - 1/800. Predicted molecular weight: 48 kDa.
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Notes |
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WB
1/100 - 1/800. Predicted molecular weight: 48 kDa. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily. -
Post-translational
modificationsSumoylated in vitro. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 15183 Mouse
- SwissProt: O88895 Mouse
- Unigene: 20521 Mouse
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Alternative names
- HD3 antibody
- HDAC 3 antibody
- HDAC3 antibody
see all
Images
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Lane 1 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/100 dilution
Lane 2 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/200 dilution
Lane 3 : Anti-HDAC3 antibody [3G6] - C-terminal (ab170199) at 1/400 dilution
All lanes : NIH 3T3 cell lysate
Predicted band size: 48 kDa
Exposure time: 30 secondsNIH 3T3 cell lysate was separated by 10% SDS PAGE and blotted onto nitrocellulose membrane. The membrane was blocked in 3% NFDM in PBS-T for 1h at RT and incubated at 4°C O/N with ab170199.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab170199 has not yet been referenced specifically in any publications.