HDAC3+NCOR2 complex protein (Tagged-His Tag) (ab42631)

Product Name 

HDAC3+NCOR2 complex protein (Tagged-His Tag)

Product type 

Proteins and Peptides

Peptide 

Complex of human HDAC3 (GenBank Accession No. NM_003883), full length with C-terminal His tag, MW= 49.7 kDa, and human NCOR2 (amino acid 395-489) GenBank Accession No. NM_006312), N-terminal proprietary tag, co-expressed in baculovirus expression system.

Specificity 

Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.

Tested applications 
(see key)

Inhib

Application notes 
(see key)

Recommended dilutions
Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Research areas 

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Chromatin and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors

Relevance 

HDAC3 is responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDAC3 probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. It is required to repress transcription of the POU1F1 transcription factor.
NCOR2 mediates the transcriptional repression activity of some nuclear receptors by promoting chromatin condensation, thus preventing access of the basal transcription.

Purity 

>90% by SDS-PAGE

Storage buffer 

Preservative: None
Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8

Material safety datasheet (MSDS) for this product:
Glycerol MSDS

Form 

Liquid

Storage instructions 

Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.

Notes 

Co-expressed in a Baculovirus infected Sf9 cell expression system.

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