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Products:Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Overview
Product name
HDAC3+NCOR2 complex protein (Tagged-His Tag)
Protein description
Complex of human HDAC3 (GenBank Accession No. NM_003883), full length with C-terminal His tag, MW= 49.7 kDa, and human NCOR2 (amino acid 395-489) GenBank Accession No. NM_006312), N-terminal proprietary tag, co-expressed in baculovirus expression system.
Expression host
Baculovirus
Properties
Purity
>90% by SDS-PAGE
Biological activity
Activity: 110 U/ug. One U =1 pmol/min, Assay condition: 25 mM Tris/Cl, pH8.0, 137 mM NaCl, 2.7 mM KCl, 1 mM MgCl2, and 0.1 mg/ml BSA, 30 uM Biomol substrate (Catalog number KI177), and 1 ng/ul HDAC3/NcoR2. Incubation condition: 30 min at 37ºC.
Form
Liquid
Storage instructions
Aliquot and store at -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 10% Glycerol, 50mM Tris HCl, 20mM Glutathione, 138mM Sodium chloride, pH 8
Concentration
Concentration information loading...
Additional notes
Co-expressed in a Baculovirus infected Sf9 cell expression system.
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> p53 pathway
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class II / Hda1 Class
Epigenetics and Nuclear Signaling >> Nuclear Signaling Pathways >> Nuclear Receptors >> Co-activators/co-repressors
Applications
Show applications key
Our Abpromise guarantee covers the use of ab42631 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Inhibition Assay
Application notes
Inhib: Use at an assay dependent dilution. Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Protein info
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Sumoylated in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
References for HDAC3+NCOR2 complex protein (Tagged-His Tag) (ab42631)
ab42631 has not yet been referenced specifically in any publications.
Publishing research using ab42631? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"