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Anti-HDAC3 (acetyl) antibody [EP462Y] (ab76295)

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Overview

Product name

Anti-HDAC3 (acetyl) antibody [EP462Y]
See all HDAC3 products (13) ...

Description

Rabbit monoclonal [EP462Y] to HDAC3 (acetyl)

Tested applications

WB, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Human

Does not react with

Rat

Immunogen

A synthetic peptide corresponding to residues near the N-terminus of Histone deacetylase 3.

Positive control

HeLa nuclear lysate Human breast carcinoma tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EP462Y

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - HDAC3 (acetyl) antibody [EP462Y] (ab76295)Western blot - HDAC3 (acetyl) antibody [EP462Y] (ab76295) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 (acetyl) antibody [EP462Y] (ab76295)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 (acetyl) antibody [EP462Y] (ab76295) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76295 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/2000. Det...Read more →

    WB: 1/1000 - 1/2000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250. Pe...Read more →

    IHC-P: 1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • ICC

     ICC: 1/100.

    ICC: 1/100.

Application notes

Is unsuitable for ,Flow Cyt or IP.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated in vitro.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO15379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • RPD3 2 antibody
  • HD 3 antibody
  • HD3 antibody
  • HDAC 3 antibody
  • HDAC3 antibody
  • HDAC3_HUMAN antibody
  • Histone deacetylase 3 (HD3) (RPD3-2) antibody
  • histone deacetylase 3 antibody
  • RPD 3 antibody
  • RPD3 antibody
  • RPD3-2 antibody
  • SMAP 45 antibody
  • SMAP45 antibody
see all

Database links

Anti-HDAC3 (acetyl) antibody [EP462Y] images:

  Western blot - HDAC3 (acetyl) antibody [EP462Y] (ab76295)

Western blot - HDAC3 (acetyl) antibody [EP462Y] (ab76295)

Anti-HDAC3 (acetyl) antibody [EP462Y] (ab76295) at 1/2000 dilution + HeLa cell lysate at 10 µg

Secondary
goat anti-rabbit HRP at 1/2000 dilution

Predicted band size : 49 kDa
Observed band size : 49 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 (acetyl) antibody [EP462Y] (ab76295)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 (acetyl) antibody [EP462Y] (ab76295)

ab76295, at a 1/100-1/250 dilution, staining human HDAC3 in breast carcinoma, by Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

References for Anti-HDAC3 (acetyl) antibody [EP462Y] (ab76295)

ab76295 has not yet been referenced specifically in any publications.

Publishing research using ab76295? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"