Anti-HDAC3 antibody [3A7B5] (ab76364)

Overview

• Product nameAnti-HDAC3 antibody [3A7B5]See all HDAC3 primary antibodies ...
• Description
  Mouse monoclonal [3A7B5] to HDAC3
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  Purified truncated recombinant Trx-Macrophage Scavenger Receptor I (224-428) expressed in E.Coli strain BL21 (DE3).

• Positive controlHuman oesophagus cancer and breast carcinoma tissues; and HeLa cell lysate.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.03% Sodium Azide
  Constituents: Ascites
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number3A7B5
• IsotypeIgG1
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • HDACs

  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Other

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Cardiovascular
  • Heart
  • Hypertrophy
  • Other

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

Applications

Target

• FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
• Tissue specificityWidely expressed.
• Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
• Post-translational
  modificationsSumoylated in vitro.
• Cellular localizationNucleus.

Target information above from: UniProt accession O15379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 8841 Human
  • Omim: 605166 Human
  • SwissProt: O15379 Human
  • Unigene: 519632 Human

• Alternative names
  HD 3 antibodyHD3 antibodyHDAC 3 antibody

  HDAC3 antibodyHDAC3_HUMAN antibodyHistone deacetylase 3 (HD3) (RPD3-2) antibodyhistone deacetylase 3 antibodyRPD 3 antibodyRPD3 2 antibodyRPD3 antibodyRPD3-2 antibodySMAP 45 antibodySMAP45 antibody

  see all

Anti-HDAC3 antibody [3A7B5] images

• 

  Western blot - HDAC3 antibody [3A7B5] (ab76364)
  All lanes : Anti-HDAC3 antibody [3A7B5] (ab76364) at 1/500 dilution
  
  Lane 1 : truncated Trx-HDAC3
  Lane 2 : full length HDAC3-hIgGFc
  Lane 3 : HeLa cell lysate
  
  
  Predicted band size : 50 kDa
  Observed band size : 40,50,80 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Scavenger Receptor I antibody [3A7B5] (ab76364)
  ab76364 at 1/200 dilution staining Macrophage Scavenger Receptor I in human oesophagus cancer by Immunohistochemistry, paraffin-embedded tissue with DAB staining showing nuclear location.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Macrophage Scavenger Receptor I antibody [3A7B5] (ab76364)
  ab76364 at 1/200 dilution staining Macrophage Scavenger Receptor I in human breast carcinoma by Immunohistochemistry, paraffin-embedded tissue with DAB staining showing nuclear location.