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Anti-HDAC3 antibody [7G6C5] (ab76359)

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Overview

Product name

Anti-HDAC3 antibody [7G6C5]
See all HDAC3 products (13) ...

Description

Mouse monoclonal [7G6C5] to HDAC3

Tested applications

WB, ELISA, Sandwich ELISAmore details

Cross reactivity

Reacts with

Human

Immunogen

Purified truncated recombinant Trx-HDAC3 (corresponding to amino acids 224-428 of Human HDAC3) expressed in E.Coli strain BL21 (DE3).

Positive control

HeLa cell lysate. Truncated Trx-HDAC3 recombinant protein. Full length HDAC3-hIgGFc transfected CHO-K1 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.03% Sodium Azide
Constituents: Ascites

Concentration

Concentration information loading...

Purity

Ascites

Clonality

Monoclonal

Clone number

7G6C5

Isotype

IgG2a

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Sandwich ELISA - HDAC3 antibody [7G6C5] (ab76359)Sandwich ELISA - HDAC3 antibody [7G6C5] (ab76359) image (enlarge)

  • Western blot - HDAC3 antibody [7G6C5] (ab76359)Western blot - HDAC3 antibody [7G6C5] (ab76359) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated in vitro.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO15379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • RPD3 2 antibody
  • HD 3 antibody
  • HD3 antibody
  • HDAC 3 antibody
  • HDAC3 antibody
  • HDAC3_HUMAN antibody
  • Histone deacetylase 3 (HD3) (RPD3-2) antibody
  • histone deacetylase 3 antibody
  • RPD 3 antibody
  • RPD3 antibody
  • RPD3-2 antibody
  • SMAP 45 antibody
  • SMAP45 antibody
see all

Database links

Anti-HDAC3 antibody [7G6C5] images:

  Sandwich ELISA - HDAC3 antibody [7G6C5] (ab76359)

Sandwich ELISA - HDAC3 antibody [7G6C5] (ab76359)

Standard Curve for HDAC3 (Analyte: HDAC3 protein (Tagged) (ab82071)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [7G6C5] to HDAC3 (ab76359) at 5 ug/ml and Detector Antibody Rabbit polyclonal to HDAC3 (ab63353) at 0.5 ug/ml.

  Western blot - HDAC3 antibody [7G6C5] (ab76359)

Western blot - HDAC3 antibody [7G6C5] (ab76359)

All lanes : Anti-HDAC3 antibody [7G6C5] (ab76359) at 1/500 dilution

Lane 1 : Truncated Trx-HDAC3 recombinant protein
Lane 2 : Full length HDAC3-hIgGFc transfected CHO-K1 cell lysate
Lane 3 : HeLa cell lysate


Predicted band size : 49 kDa
Observed band size : 41,45,85 kDa (why is the actual band size different from the predicted?)

Protocols

  • Protocol Booklet

    • References for Anti-HDAC3 antibody [7G6C5] (ab76359)

    ab76359 has not yet been referenced specifically in any publications.

    Publishing research using ab76359? Please let us know so that we can cite the reference in this datasheet

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"