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Overview
Product name
Anti-HDAC3 antibody - ChIP Grade
See all HDAC3 products (12) ...
Description
Rabbit polyclonal to HDAC3 - ChIP Grade
Tested applications
IP, ChIP, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Chicken, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: AKTVAYFYDPDVGNFH, corresponding to N terminal amino acids 2-17 of Human HDAC3
AKTVAYFYDP DVGNFH
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
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Western blot - HDAC3 antibody (ab47237)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab47237 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution.
ChIP: Use at an assay dependent dilution. (Refer to Caretti et al (2003, Fig.3) and Imbriano (2005, Fig 7) for details. )
WB: Use a concentration of 2 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 49 kDa).
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Sumoylated in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RPD3 2 antibody
- HD 3 antibody
- HD3 antibody
see all
Database links
- Clone ID: BC000614 Human
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Entrez Gene: 84578 Rat
- Entrez Gene: 393965 Zebrafish
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
see all
Anti-HDAC3 antibody - ChIP Grade images:
Western blot - HDAC3 antibody (ab47237)
Anti-HDAC3 antibody - ChIP Grade (ab47237) at 2 µg/ml + HeLa cell lysate
Predicted band size : 49 kDa
Observed band size : 49 kDa
References for Anti-HDAC3 antibody - ChIP Grade (ab47237)
This product has been referenced in:
- Imbriano Cet al. Direct p53 transcriptional repression: in vivo analysis of CCAAT-containing G2/M promoters. Mol Cell Biol 25:3737-51 (2005).Read more (PubMed: 15831478) »
- Doyle K & Fitzpatrick FA Redox signaling, alkylation (carbonylation) of conserved cysteines inactivates class I histone deacetylases 1, 2, and 3 and antagonizes their transcriptional repressor function. J Biol Chem 285:17417-24 (2010). WB.Read more (PubMed: 20385560) »
See all 2 publications for this product
Publishing research using ab47237? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"