|
|
|
|
ChIP - HDAC3 antibody - ChIP Grade (ab7030)
(enlarge)
|
|
Immunofluorescence - HDAC3 antibody - ChIP Grade (ab7030)
(enlarge)
|
|
Western blot - HDAC3 antibody - ChIP Grade (ab7030)
(enlarge)
|
|
|
|
|
Product Name
|
HDAC3 antibody - ChIP Grade
|
| |
See all HDAC3 antibodies (12)...
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to HDAC3 - ChIP Grade
|
|
Immunogen
|
Synthetic peptide: C-NEFYDGDHDNDKESDVEI conjugated to KLH, corresponding to amino acids 411-428 of Human HDAC3.
BLAST 'C-NEFYDGDHDNDKESDVEI' with  or 
|
|
Reacts with
(species key)
|
Hu, Ms
|
|
Specificity
|
Anti-Histone Deacetylase 3 specifically recognizes histone deacetylase 3 by immunoblotting and immunoprecipitation (50 kD).
|
|
Epitope
|
The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
|
|
Tested applications
(see key)
|
ChIP, Dot, ICC/IF, IHC-P, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ChIP: Use 2µl for 2x10^6 cells.
Dot: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IHC-P: 1/500.
IP: 1/2500.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Nuclear
|
|
Research areas
|
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Chromatin and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
|
|
Relevance
|
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
IgG fraction
|
|
Storage buffer
|
0.01M PBS pH7.4, 15mM Na azide Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
|
|
Form
|
Liquid
|
|
Concentration
|
Batch dependent within range: 11.70 - 13.30 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC3 antibody - ChIP Grade is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for HDAC3 antibody - ChIP Grade images, references, products related to ab7030 and other tools.
|
| |
HDAC3 antibody - ChIP Grade images:
|
 ChIP - HDAC3 antibody - ChIP Grade (ab7030)
ab7030 used in ChIP
Sonicated Chromatin prepared from untreated (UI) or 17beta-estradiol (E2) treated MCF7 cells was subjected to the ChIP procedure with ab7030 to HDAC3 and the immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are nomalized over inputs). The primers are designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 2 µl of ab7030 and 2x106 cells were used in each ChIP experiment. Sylvain Daujat
|
| |
Immunofluorescence - HDAC3 antibody - ChIP Grade (ab7030)
Cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/5000 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in TBS-T. Coverslips were then counterstained with DAPI in TBS-T for 1-2 minutes, TBS-T was then added and the coverslips mounted. Red indicates staining by ab7030, blue by DAPI. A small amount of cytoplasmic staining was seen with ab7030. Michael Mancini, Baylor College of Medicine
|
| |
Western blot - HDAC3 antibody - ChIP Grade (ab7030)
All lanes : HDAC3 antibody - ChIP Grade (ab7030) at 1/10000 dilution
Lane 1 : Whole cell lysate from human HEK293 cell line
Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC3 gene silencing shRNA
Lysates/proteins at 20 µg per lane.
Secondary
HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 49 kDa
Exposure time : 10 seconds This image is courtesy of an anonymous Abreview
See Abreview
|
| |
References for HDAC3 antibody - ChIP Grade (ab7030)
|
|
This product has been used in: (two most recent references)
Xu C et al. Chemical probes identify a role for histone deacetylase 3 in Friedreich's ataxia gene silencing. Chem Biol 16:980-9 (2009). WB; Human. PubMed: 19778726
Fritah S et al. Heat-shock factor 1 controls genome-wide acetylation in heat-shocked cells. Mol Biol Cell 20:4976-84 (2009). WB. PubMed: 19793920
See all 17 publication references for this product.
If you publish research using ab7030 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to HDAC3
|
| |
HDAC3 antibody - ChIP Grade - more information
|
Customer reviews (feedback) regarding HDAC3 antibody - ChIP Grade |
Customer FAQs regarding HDAC3 antibody - ChIP Grade |
Protocols for HDAC3 antibody - ChIP Grade |
Price and availability of products related to HDAC3 antibody - ChIP Grade |
| |
HDAC3 antibody - ChIP Grade - related products:
|
|
|
| |
HDAC3 antibody - ChIP Grade - other tools:
|
Contact Abcam with a Technical Enquiry about HDAC3 antibody - ChIP Grade |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
