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Anti-HDAC3 antibody [Y415] (ab32369)

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1 question for ab32369

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Question 1

Friday 28-September-2007

I am seeing cytoplasmic staining as well as nuclear staining in ICC, and a signal at the expected MW in Western blots of cytosolic cell fractions. Blots for lamin confirm that the fractionation procedure was correct; there is no lamin detected in the cytosolic fraction.

ANSWER:

  

Thank you for your enquiry.

I understand your concern. There does, however, appear to be some evidence that HDAC3 can be found in the cytosol. Please see Figure 6 in the paper found at the following link:

http://www.pubmedcentral.nih.gov/articlerender.fcgi?artid=1618574

Am J Pathol. 2004 August; 165(2): 553–564.

There may be other papers referenced in this article that discuss the same observation.

I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

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