Overview

  • Product nameAnti-HDAC3 antibody [Y415]See all HDAC3 primary antibodies ...
  • Description
    Rabbit monoclonal [Y415] to HDAC3
  • SpecificityThis antibody may detect splice isoform 2 (RPD3-2A).
  • Tested applicationsWB, IHC-P, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    A synthetic peptide corresponding to residues in the C-terminal region of human Histone deacetylase 3.

  • Positive control
    • K562 cell lysate; HeLa cells; human ovary carcinoma
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab32369 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
IHC-P
ICC/IF
IP
  • Application notesICC/IF: 1/250 - 1/500.
    IHC-P: 1/250 - 1/500.
    IP: 1/50.
    WB: 1/5000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

    Is unsuitable for FACS.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • FunctionResponsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
    • Tissue specificityWidely expressed.
    • Sequence similaritiesBelongs to the histone deacetylase family. HD type 1 subfamily.
    • Post-translational
      modifications
      Sumoylated in vitro.
    • Cellular localizationNucleus.
    • Information by UniProt
    • Database links
    • Alternative names
      • HD 3 antibody
      • HD3 antibody
      • HDAC 3 antibody
      • HDAC3 antibody
      • HDAC3_HUMAN antibody
      • Histone deacetylase 3 (HD3) (RPD3-2) antibody
      • histone deacetylase 3 antibody
      • RPD 3 antibody
      • RPD3 2 antibody
      • RPD3 antibody
      • RPD3-2 antibody
      • SMAP 45 antibody
      • SMAP45 antibody
      see all

    Anti-HDAC3 antibody [Y415] images

    • Anti-HDAC3 antibody [Y415] (ab32369) at 1/10000 dilution + K562 cell lysate

      Predicted band size : 49 kDa
      Observed band size : 49 kDa
    • Paraffin-embedded human ovary carcinoma
      ab32369 at 1/250 dilution
    • Immunofluorescent staining of HeLa cells
      using ab32369 at 1/250 dilution

    References for Anti-HDAC3 antibody [Y415] (ab32369)

    This product has been referenced in:
    • Chuang MJ  et al. The HDAC inhibitor LBH589 induces ERK-dependent prometaphase arrest in prostate cancer via HDAC6 inactivation and down-regulation. PLoS One 8:e73401 (2013). WB ; Human . Read more (PubMed: 24023871) »
    • Papadopoulos P  et al. A dual reporter mouse model of the human ß-globin locus: applications and limitations. PLoS One 7:e51272 (2012). WB ; Mouse . Read more (PubMed: 23272095) »

    See all 5 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Mouse Tissue sections (embryo)
    Specification embryo
    Fixative Formaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citrate based, high pH (Vector lab)
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Username

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    Submitted Feb 02 2012

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Lymph node)
    Specification Lymph node
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Citric buffer, pH6
    Permeabilization No
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 20°C
    Username

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    Verified customer

    Submitted Jun 01 2011

    Thank you for your enquiry. I understand your concern. There does, however, appear to be some evidence that HDAC3 can be found in the cytosol. Please see Figure 6 in the paper found at the following link: http://www.pubmedcentral.nih.gov/articl...

    Read More
    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Human Tissue sections (Breast Tumour)
    Specification Breast Tumour
    Fixative Paraformaldehyde
    Antigen retrieval step Heat mediated
    Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 20
    Username

    Abcam user community

    Verified customer

    Submitted Jul 28 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"