![Western blot - HDAC3 antibody [Y415] (ab32369) image](#ab32369WB.bmp)
![Immunohistochemistry (Paraffin-embedded sections) - HDAC3 antibody [Y415] (ab32369) image](#ab32369IHCP.bmp)
![Immunocytochemistry/ Immunofluorescence - HDAC3 antibody [Y415] (ab32369) image](#ab32369IF.bmp)
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Overview
Product name
Anti-HDAC3 antibody [Y415]
See all HDAC3 products (12) ...
Description
Rabbit monoclonal [Y415] to HDAC3
Specificity
This antibody may detect splice isoform 2 (RPD3-2A).
Tested applications
IHC-P, WB, IHC-P, ICC/IF, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
A synthetic peptide corresponding to residues in the C-terminal region of human Histone deacetylase 3.
Positive control
K562 cell lysate; HeLa cells; human ovary carcinoma
General notes
Produced under U.S. Patent No. 5,675,063.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, 0.15M Sodium chloride, 50mM Tris glycine. pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
Y415
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32369 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
ICC/IF: 1/250 - 1/500.
IHC-P: 1/250 - 1/500.
IP: 1/50.
WB: 1/5000 - 1/10000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Is unsuitable for FACS.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Sumoylated in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RPD3 2 antibody
- HD 3 antibody
- HD3 antibody
see all
Database links
- Clone ID: BC000614 Human
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Entrez Gene: 84578 Rat
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
- SwissProt: Q6P6W3 Rat
see all
Anti-HDAC3 antibody [Y415] images:
Western blot - HDAC3 antibody [Y415] (ab32369)
![Western blot - HDAC3 antibody [Y415] (ab32369)](/ps/datasheet/Images/32/ab32369/ab32369WB.bmp)
Anti-HDAC3 antibody [Y415] (ab32369) at 1/10000 dilution + K562 cell lysate
Predicted band size : 49 kDa
Observed band size : 49 kDa
Immunohistochemistry (Paraffin-embedded sections) - HDAC3 antibody [Y415] (ab32369)
![Immunohistochemistry (Paraffin-embedded sections) - HDAC3 antibody [Y415] (ab32369)](/ps/datasheet/Images/32/ab32369/ab32369IHCP.bmp)
Paraffin-embedded human ovary carcinoma
ab32369 at 1/250 dilution
Immunocytochemistry/ Immunofluorescence - HDAC3 antibody [Y415] (ab32369)
![Immunocytochemistry/ Immunofluorescence - HDAC3 antibody [Y415] (ab32369)](/ps/datasheet/Images/32/ab32369/ab32369IF.bmp)
Immunofluorescent staining of HeLa cells
using ab32369 at 1/250 dilution
References for Anti-HDAC3 antibody [Y415] (ab32369)
This product has been referenced in:
- Funato H, Oda S, Yokofujita J, Igarashi H, KurodaFasting and high-fat diet alter histone deacetylase expression in the medial hypothalamus. PLoS One 6:e18950 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21526203) »
- Knutson SKet al. Liver-specific deletion of histone deacetylase 3 disrupts metabolic transcriptional networks. EMBO J 27:1017-28 (2008). IHC-P; Mouse.Read more (PubMed: 18354499) »
See all 2 publications for this product
Publishing research using ab32369? Please let us know so that we can cite the reference in this datasheet
![HDAC3 antibody [Y415] for Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) in Human (32369)](/ps/datasheet/images/32/ab32369/HDAC3-Primary-antibodies-ab32369-2.jpg)
![HDAC3 antibody [Y415] for IHC-P in Human (32369)](/ps/Reviews/Images/ab5128_17791.jpg)
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"