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Western blot
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Western blot - HDAC3 antibody (ab16047)
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Immunocytochemistry - HDAC3 antibody (ab16047)
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Product Name
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HDAC3 antibody
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See all HDAC3 antibodies (12)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to HDAC3
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human HDAC3.
(Peptide available as ab16279.)
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Reacts with
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Hu, Ms, Rat, InMtj
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Predicted to react
(species key)
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Chk, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ICC/IF, IHC-P, WB
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Abreviews
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Application notes
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Recommended dilutions
ICC/IF: 1/100 - 1/500.
IHC-P: Use at a dilution of 1/2000 for 1 hr.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). Can be blocked with HDAC3 peptide (ab16279).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Nuclear
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Research areas
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Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Chromatin and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
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Relevance
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Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to the histone acetylase/acuc/apha family. It has histone deacetylase activity and represses transcription when tethered to a promoter. It may participate in the regulation of transcription through its binding with the zinc-finger transcription factor YY1. This protein can also down-regulate p53 function and thus modulate cell growth and apoptosis. This gene is regarded as a potential tumor suppressor gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this HDAC3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for HDAC3 antibody images, references, products related to ab16047 and other tools.
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HDAC3 antibody images:
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 Western blot - HDAC3 antibody (ab16047)
All lanes : HDAC3 antibody (ab16047) at 1 µg/ml
Lane 1 : Mouse 3T3 cell lysate
Lane 2 : Rat liver cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
ab16047 cross-reacts with Mouse 3T3 cell lysate but shows no cross-reactivity against Rat liver lysate.
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Immunocytochemistry - HDAC3 antibody (ab16047)
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16047 (1/100). The antibody clearly stained the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification. Darin McDonald, Cross Cancer Institute
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Immunocytochemistry - HDAC3 antibody (ab16047)
Interphase HeLa cells incubated with ab16047 (1/500). The antibody shows predominantly nuclear staining. The ab16047 staining is shown in green. The cells were counterstained with DAPI (red). Kirk McManus, University of British Columbia
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab16047)
ab16047 staining HDAC3 in human breast cancer tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary. This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
See Abreview
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References for HDAC3 antibody (ab16047)
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This product has been used in:
Bhaskara S et al. Deletion of histone deacetylase 3 reveals critical roles in S phase progression and DNA damage control. Mol Cell 30:61-72 (2008). WB; Mouse. PubMed: 18406327
If you publish research using ab16047 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to HDAC3
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HDAC3 antibody - more information
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Customer reviews (feedback) regarding HDAC3 antibody |
Customer FAQs regarding HDAC3 antibody |
Protocols for HDAC3 antibody |
Price and availability of products related to HDAC3 antibody |
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HDAC3 antibody - related products:
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HDAC3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about HDAC3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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