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Overview
Product name
Anti-HDAC3 antibody
See all HDAC3 products (12) ...
Description
Rabbit polyclonal to HDAC3
Tested applications
IHC-P, ICC/IF, ICC, IF, WB, IPmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Indian Muntjac
Predicted to work with
Chicken, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human HDAC3.
(Peptide available as ab16279.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab16047 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent concentration.
ICC/IF: Use at an assay dependent concentration.
ICC: 1/100 - 1/500.
IF
IF: 1/100 - 1/500.IF: 1/100 - 1/500.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with HDAC3 peptide (ab16279).
IP: Use at an assay dependent concentration.
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Sumoylated in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RPD3 2 antibody
- HD 3 antibody
- HD3 antibody
see all
Database links
- Clone ID: BC000614 Human
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Entrez Gene: 84578 Rat
- Entrez Gene: 393965 Zebrafish
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
see all
Anti-HDAC3 antibody images:
Western blot
All lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/ml
Lane 1 : HeLa nuclear lysate
Lane 2 : HeLa whole cell lysate
Lane 3 : A431 cell lysate
Lane 4 : Jurkat cell lysate
Lane 5 : HEK293 cell lysate
Lane 6 : HeLa nuclear lysate with HDAC3 peptide (ab16279) at 1 µg/ml
Lane 7 : HeLa whole cell lysate with HDAC3 peptide (ab16279) at 1 µg/ml
Lane 8 : A431 cell lysate with HDAC3 peptide (ab16279) at 1 µg/ml
Lane 9 : Jurkat cell lysate with HDAC3 peptide (ab16279) at 1 µg/ml
Lane 10 : HEK293 cell lysate with HDAC3 peptide (ab16279) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
Western blot - HDAC3 antibody (ab16047)
All lanes : Anti-HDAC3 antibody (ab16047) at 1 µg/ml
Lane 1 : Mouse 3T3 cell lysate
Lane 2 : Rat liver cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 49 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 seconds
ab16047 cross-reacts with Mouse 3T3 cell lysate but shows no cross-reactivity against Rat liver lysate.
Immunocytochemistry - HDAC3 antibody (ab16047)
SK-N-SH cells were fixed in 4% paraformaldehyde, permeabilized in 0.5% Triton X-100 and incubated for 1 hour with ab16047 (1/100). The antibody clearly stained the nucleus (red). The cells were counterstained with DAPI (blue). 100x magnification.
Darin McDonald, Cross Cancer Institute
Immunocytochemistry - HDAC3 antibody (ab16047)
Interphase HeLa cells incubated with ab16047 (1/500). The antibody shows predominantly nuclear staining. The ab16047 staining is shown in green. The cells were counterstained with DAPI (red).
Kirk McManus, University of British Columbia
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab16047)
ab16047 staining HDAC3 in human breast cancer tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval in Citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block then 10 minutes/protein block) for 15 minutes at 20°C. The primary antibody was diluted, 1/2000 and incubated with sample for 45 minutes at 20°C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary.
This image is courtesy of an Abreview submitted by Antibody Solutions Ltd.
Immunoprecipitation - Anti-HDAC3 antibody (ab16047)
HDAC3 was immunoprecipitated using 0.5mg Hela whole cell extract, 5ug of Rabbit polyclonal to HDAC3 and 50µl of protein G magnetic beads (lane 1). The antibody was incubated with the Protein G beads for 10min under agitation. No antibody was added to the control (lane 2). Hela whole cell extractdiluted in RIPA buffer was added to each sample and incubated for 10min under agitation. Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab16047. Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697). Band: 49kDa: HDAC3.
References for Anti-HDAC3 antibody (ab16047)
This product has been referenced in:
- Samant SAet al. HDAC3-dependent Reversible Lysine Acetylation of Cardiac Myosin Heavy Chain Isoforms Modulates Their Enzymatic and Motor Activity. J Biol Chem 286:5567-77 (2011).Read more (PubMed: 21177250) »
- Brunmeir Ret al. Epigenetic regulation of a murine retrotransposon by a dual histone modification mark. PLoS Genet 6:e1000927 (2010). WB; Mouse.Read more (PubMed: 20442873) »
See all 5 publications for this product
Publishing research using ab16047? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"