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Anti-HDAC3 antibody (ab61216)

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Overview

Product name

Anti-HDAC3 antibody
See all HDAC3 products (12) ...

Description

Rabbit polyclonal to HDAC3

Specificity

Detects endogenous levels of total HDAC3 protein.

Tested applications

ELISA, ICC/IF, IHC-P, WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat

Immunogen

Synthetic peptide derived from C-terminal of human HDAC3

Positive control

COS7 cells, extracts from HepG2 cells, human breast carcinoma tissue

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - HDAC3 antibody (ab61216)Western blot - HDAC3 antibody (ab61216) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab61216)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab61216) image (enlarge)

  • Immunocytochemistry/ Immunofluorescence - HDAC3 antibody (ab61216)Immunocytochemistry/ Immunofluorescence - HDAC3 antibody (ab61216) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab61216 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     ELISA: 1/5000

    ELISA: 1/5000

  • 1 Image

    ICC/IF

     ICC/IF: 1/500 - 1/1000.

    ICC/IF: 1/500 - 1/1000.

  • 1 Image

    IHC-P

     IHC-P: 1/50 - 1/100.

    IHC-P: 1/50 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/500 - 1/1000.Detec...Read more →

    WB: 1/500 - 1/1000.Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated in vitro.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO15379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • RPD3 2 antibody
  • HD 3 antibody
  • HD3 antibody
  • HDAC 3 antibody
  • HDAC3 antibody
  • HDAC3_HUMAN antibody
  • Histone deacetylase 3 (HD3) (RPD3-2) antibody
  • histone deacetylase 3 antibody
  • RPD 3 antibody
  • RPD3 antibody
  • RPD3-2 antibody
  • SMAP 45 antibody
  • SMAP45 antibody
see all

Database links

see all

Anti-HDAC3 antibody images:

  Western blot - HDAC3 antibody (ab61216)

Western blot - HDAC3 antibody (ab61216)

All lanes : Anti-HDAC3 antibody (ab61216) at 1/500 dilution

Lane 1 : extracts from HepG2 cells
Lane 2 : extracts from HepG2 cells with immunizing peptide


Predicted band size : 49 kDa
Observed band size : 49 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab61216)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab61216)

Ab61216 at 1/50-1/100 dilution staining human breast carcinoma tissue without (left) and with (right) immunizing peptide; paraffin embedded.

  Immunocytochemistry/ Immunofluorescence - HDAC3 antibody (ab61216)

Immunocytochemistry/ Immunofluorescence - HDAC3 antibody (ab61216)

Ab61216 at 1/500-1/1000 dilution staining COS7 cells without (left) and with (right) immunizing peptide.

References for Anti-HDAC3 antibody (ab61216)

ab61216 has not yet been referenced specifically in any publications.

Publishing research using ab61216? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"