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Product name
Anti-HDAC3 antibody
See all HDAC3 products (13) ...
Description
Rabbit polyclonal to HDAC3
Tested applications
Sandwich ELISA, ELISA, ICC/IF, IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthesized non-phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424.
Positive control
Human skin tissue. Extracts from NIH/3T3 cells. HeLa cells, treated with TNF-a.
Properties
Form
Liquid
Storage instructions
Store at -20°C. Stable for 12 months at -20°C
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation
Applications
Show applications key
Our Abpromise guarantee covers the use of ab63353 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
sELISA: Use a concentration of 0.5 µg/ml. Can be paired for Sandwich ELISA with Mouse monoclonal [7G6C5] to HDAC3 (ab76359). For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [7G6C5] to HDAC3 (ab76359) as Capture.
ELISA
ELISA: 1/5000.ELISA: 1/5000.
ICC/IF: 1/500 - 1/1000.
IHC-P: 1/50 - 1/100.
WB: 1/500 - 1/1000. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
Target
Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
Tissue specificity
Widely expressed.
Sequence similarities
Belongs to the histone deacetylase family. HD type 1 subfamily.
Post-translational
modifications
Sumoylated in vitro.
Cellular localization
Nucleus.
Target information above from: UniProt accessionO15379
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- RPD3 2 antibody
- HD 3 antibody
- HD3 antibody
see all
Database links
- Entrez Gene: 8841 Human
- Entrez Gene: 15183 Mouse
- Omim: 605166 Human
- SwissProt: O15379 Human
- SwissProt: O88895 Mouse
- Unigene: 519632 Human
- Unigene: 20521 Mouse
Anti-HDAC3 antibody images:
Sandwich ELISA - HDAC3 antibody (ab63353)
Standard Curve for HDAC3 (Analyte: HDAC3 protein (Tagged) (ab82071)); dilution range 1 pg/ml to 1 ug/ml using Capture Antibody Mouse monoclonal [7G6C5] to HDAC3 (ab76359) at 5 ug/ml and Detector Antibody Rabbit polyclonal to HDAC3 (ab63353) at 0.5 ug/ml.
Western blot - HDAC3 antibody (ab63353)
All lanes : Anti-HDAC3 antibody (ab63353) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells, without immunizing peptide
Lane 2 : extracts from NIH/3T3 cells, with immunizing peptide
Predicted band size : 49 kDa
Observed band size : 49 kDa
Additional bands at : 100 kDa. We are unsure as to the identity of these extra bands.
The amount of positive control loading for the WB is 5-30 ug of total protein. The amount of the peptide for the WB is 5-10 ug.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HDAC3 antibody (ab63353)
Immunohistochemical analysis of HDAC3 expression in paraffin-embedded human skin tissue, using ab63353 at 1/50 dilution. Right: sample treated with immunising peptide. Left: untreated sample.
Immunocytochemistry/ Immunofluorescence - HDAC3 antibody (ab63353)
Immunofluorescence analysis of HDAC3 expression in HeLa cells, using ab63353 at 1/500 dilution. Left: Cells treated with TNF-a (20nM, 15mins). Right: Untreated cells.
Protocols
References for Anti-HDAC3 antibody (ab63353)
This product has been referenced in:
- Razidlo DF et al. Histone deacetylase 3 depletion in osteo/chondroprogenitor cells decreases bone density and increases marrow fat. PLoS One 5:e11492 (2010). WB; Mouse.Read more (PubMed: 20628553) »
See 1 publication for this product
Publishing research using ab63353? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"