You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Anti-HDAC3 (phospho S424) antibody (ab61056)

CodeSizePriceAbpointsAvailability
    
Add to basket
 

  • -

  •   
  •   
  •   

  •  

  •  
  •  

  •  

  •  
Updating...

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »

Overview

Product name

Anti-HDAC3 (phospho S424) antibody
See all HDAC3 products (13) ...

Description

Rabbit polyclonal to HDAC3 (phospho S424)

Specificity

ab61056 detects endogenous levels of HDAC3 only when phosphorylated at serine 424.

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Synthetic phosphopeptide derived from human HDAC3 around the phosphorylation site of serine 424 (KESpDV).

Positive control

NIH/3T3 cell extract.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Purification notes

ab61056 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Clonality

Polyclonal

Isotype

IgG

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Metabolic signaling pathways >> Lipid and lipoprotein metabolism >> Lipid metabolism
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Metabolism of lipids and lipoproteins
Cardiovascular >> Heart >> Hypertrophy >> Other
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation >> HDACs >> Class I
Stem Cells >> Signaling Pathways >> Wnt >> HDACs
Epigenetics and Nuclear Signaling >> Chromatin Modifying Enzymes >> Acetylation

  • Western blot - HDAC3 (phospho S424) antibody (ab61056)Western blot - HDAC3 (phospho S424) antibody (ab61056) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab61056 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.

Tissue specificity

Widely expressed.

Sequence similarities

Belongs to the histone deacetylase family. HD type 1 subfamily.

Post-translational
modifications

Sumoylated in vitro.

Cellular localization

Nucleus.

Target information above from: UniProt accessionO15379 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • RPD3 2 antibody
  • HD 3 antibody
  • HD3 antibody
  • HDAC 3 antibody
  • HDAC3 antibody
  • HDAC3_HUMAN antibody
  • Histone deacetylase 3 (HD3) (RPD3-2) antibody
  • histone deacetylase 3 antibody
  • RPD 3 antibody
  • RPD3 antibody
  • RPD3-2 antibody
  • SMAP 45 antibody
  • SMAP45 antibody
see all

Database links

Anti-HDAC3 (phospho S424) antibody images:

  Western blot - HDAC3 (phospho S424) antibody (ab61056)

Western blot - HDAC3 (phospho S424) antibody (ab61056)

All lanes : Anti-HDAC3 (phospho S424) antibody (ab61056) at 1/500 dilution

Lane 1 : NIH/3T3 cell extract with no phosphopeptide
Lane 2 : NIH/3T3 cell extract with phosphopeptide


Predicted band size : 49 kDa
Observed band size : 49 kDa

References for Anti-HDAC3 (phospho S424) antibody (ab61056)

ab61056 has not yet been referenced specifically in any publications.

Publishing research using ab61056? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"